Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx

... 2002 Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low ... B.W. (1993) Enzymatic hydro- lysis of whey proteins. In uence of heat treatment of alpha-lac- talbumin and beta-lactoglobulin on their p...

Ngày tải lên: 21/02/2014, 15:20

... protease cathepsin D, as indicated by the following observa- tions: (a) the inhibitor profile of the endosomal ETA-degrading activity was very similar to that of cathepsins B and D [30]; and (b) the ... determined. Finally, we assign an important role to the endosomal acidic cathep- sins B and D in increasing the in vitro transfer of the ADP-ribosyl moiety of NA...

Ngày tải lên: 18/02/2014, 04:20

... visualized by SOD activity staining may have been derived from further interactions of the fusion protein GST domains (Fig. 2B). Although not visualized in these zymograms, all of the fusion proteins ... combination of key residues. Further analysis of these and other mutant SODs is currently underway. ACKNOWLEDGEMENTS We are indebted to G. Peplow, F. Yamakura and T. Matsu...

Ngày tải lên: 21/02/2014, 01:21

... appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... several lines of evidence point to the presence of the thiyl radical in the aerobic system. First, the complexes are inactivated by 2-oxo acid and CoA in...

Ngày tải lên: 21/02/2014, 01:21

... model the C-terminal domain of SmGSTO due to the low rate of identity between the two proteins in this region. However, as the active sites of the GSTO1-1 are located at the N-terminal domain, ... are in line with the high GSH K m value obtained for SmGSTO and the strong inhibitory effect of S-hexyl glutathione on the enzyme activity. The preference of...

Ngày tải lên: 21/02/2014, 01:21

... consump- tion. On the other hand, the laccase/TEMPO system performs the selective oxidation of the hydroxymethyl groups of the substrate to aldehydes. Both with laccase alone and with the laccase/HPI system, ... promotes the formation of the same phenoxy radical intermediate as laccase, yields the same products as the enzyme alone, namely, ring-opening and...

Ngày tải lên: 21/02/2014, 01:21

... N-terminal of the proteinase domain (see Fig. 2 or 3). The proteinase domain of VPR additionally contains Cys277 and Cys281, and Cys351 and Cys362 in the C-terminal domain, that suppo- sedly also ... high homology to proteinases of the proteinase K family of subtilisin-like proteinases. With respect to the enzyme characteristics compared in this study the prope...

Ngày tải lên: 21/02/2014, 01:21

... NAMI-A-mediated inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2. Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/2 phosphoryla- tion activity ... as an internal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product were normalized by comparison with the level...

Ngày tải lên: 21/02/2014, 01:21

... mutated negatively and positively charged residues on the proposed interaction site of plastocyanin with cytochrome f and analysed the reaction of these mutants with the soluble redox-active domain of cytochrome ... Further- more, the type I copper protein plastocyanin can be replaced by cytochrome c 6 , a redox protein of similar size but entirely different foldin...

Ngày tải lên: 21/02/2014, 01:21

... aminoglycoside, kanamycin, the a minocyclitol glycoside, streptomycin, t he penicillins, ampicillin and penicillin G, the aminocyclitol, spectinomycin, tetracycline and Cl-tetracycline, and the macrolide, erythromycin) ... ata con®rmed the possible binding of Cl-tetracycline a nd streptomycin to the pre-mRNA. With Cl-tetracycline and s treptomycin, the preincubation re...

Ngày tải lên: 21/02/2014, 15:20