Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
... many protein folding and traffic processes i n the cell. The main compo- nent of the system is DnaK, a t wo-domain protein with a C-terminal domain responsible for the binding of unfolded hydrophobic ... N-terminal domain, which binds ATP. This protein can bind and release peptides (in the Ct domain) in a cycle driven by nucleotide hydrolysis and exchange (in the Nt domain). The stru...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide docx
... (2–5)A synthesizing activity in S. domuncula. Despite the initially high (2–5)A synthesizing activity in tissue of G. cydonium (aquarium animals), the incubation with LPS caused significant increase of ... he only species with (2–5)A synthesizing activity within the p hylum Porifera. We have identified (2–5)A synthesizing activity in different marine sponges, i ncluding S. domuncu...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... decrease in the activity for the 11b-hydroxylase, CYP11B1. Intriguingly, however, the 11b-hydroxylase activity of the aldosterone synthase CYP11B2 was basically unaffected whereas the 18-hydroxy- lation ... major mineralocorticoid by means of an 11b-hydroxylation and an 18-hydroxylation/oxidation of 11-deoxycorticosterone. Thus, the proteins of the CYP11B subfamily catalysing the la...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... properties. In this work we took advantage of the specific labeling with PLP of Lys288 in E. coli PEP carboxykinase to gain insight into conformational alterations of the protein upon ligand binding in ... domain movements in the protein. In this work, the interaction of several substrates and ligands with E. coli PEP carboxykinase has been studied in the phos- phopyr...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... constitute a class of structurally equivalent metalloenzymes prevalent in prokaryotes and in eukaryotic mitochondria, respectively. They exhibit a very high degree of homology in both sequence ... manganese. Selectivity of the proteins for their metal cofactor has been demonstrated in vivo [3] and although apoenzymes of each type of SOD may be reconstituted by the addition...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... the thiyl radical of the lipoyl residue should efficiently interact with the E1 catalytic intermediate, as the lipoyl-bearing domain of E2 is designed for this interaction (react...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... resin was washed four times with 250 lLof 50 m M Hepes, pH 8.0, and eluted by adding 10 lLofthe same buffer containing 5 m M GSH, GSSG or S-hexyl GSH. Ten microliters of the original protein, binding ... are in line with the high GSH K m value obtained for SmGSTO and the strong inhibitory effect of S-hexyl glutathione on the enzyme activity. The preference of SmGSTO for more hydro...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... 2,2,6,6- tetramethylpiperidin-1-yloxy free radical (TEMPO) as mediators, since they greatly differ both in their mechan- ism of action and in their specificity. In fact, HPI is representative of a mediator ... activity The activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6- sulfonate) (ABTS) to ABTS radical cation (A...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... identical inactivation behaviour, indicating that the same disulfide(s) are involved in maintaining the integrity of active protein structure in both wild-type and recombinant VPR. The wild-type and ... According to our homology model only one of the three extra glycines in the cold-adapted proteinase is located in a loop region of the protein. Thus an increased number of Ôflexi...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... as an internal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product were normalized by comparison with the levels of radioactivity incorporated ... inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2. Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/2 phosphoryla- tion a...
Ngày tải lên: 21/02/2014, 01:21