Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot

... t han by tetracycline (Fig. 2). The extent of splicing inhibition by tetracycline is n egligible (Fig. 2). The sixfold stron ger splicing inhibition by Cl-tetracycline may be explained by the ... on in vitro splicing of a eukaryotic nuclear pre-mRNA (b-globin). Of the eight antibiotics studied, erythromycin, Cl-tetracycline and streptomycin were identi®ed as...

Ngày tải lên: 21/02/2014, 15:20

... and ultimately abolished, the increase in c-myc gene expression elicited by PMA in serum-free cells. These results suggest that inhibition of MEK/ERK signaling by NAMI-A may have an important role in modulating ... be lying upstream of ERK1/2. Recent observations indicate that activation of Raf by PMA may trigger the same signaling pathway as oncogenic Raf, or Raf activati...

Ngày tải lên: 21/02/2014, 01:21

... results obtained when a-synuclein was incubated in the absence of MPTP. When a-synuclein was incubated alone in the presence of dopamine, it led to inhibition of fibrillation, probably by the accu- mulation ... occur in the brain include selective loss of dopaminergic neurons in substantia nigra pars compacta and appearance of Lewy bodies consisting of aggregated protei...

Ngày tải lên: 14/02/2014, 19:20

... 1.2 mL of water. l-[ 3 H]Citrulline was quantified by liquid scintillation spectroscopy. The retention of l-[ 3 H]arginine by the column was greater than 98%. Enzyme activity was determined as ... Reiter RJ (2005) Anti -in ammatory actions of melatonin and its metabolites, N 1 -acetyl- N 2 -formyl-5-methoxykynuramine (aFMK) and (N 1 - acetyl-5-methxykynuramine (AMK), in macrophages...

Ngày tải lên: 19/02/2014, 00:20

... (1996) In vitro inactivation of mammalian methionine synthase by nitric oxide. Eur J Clin Invest 26, 167–170. 15 Alston TA (1991) Inhibition of vitamin B12-dependent methionine biosynthesis by chloroform ... or cofactor. In detail, the N-terminal module is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the third domain binds CH 3 -Cbl;...

Ngày tải lên: 19/02/2014, 05:20

... (tetrabutylammonium), cyclic aliphatic (1-methylpyrrolidine; N,N-dimethyl- cyclohexylamine) or aromatic [2,4,6-tris(dimethyl- aminoethyl)phenol] chains, that might in principle better fill the hydrophobic binding ... occupation of high-affinity binding sites by choline triggers the dimerization of C-LytA [21]. The effect of bicyclic amines on C-LytA oligomerization was ana- lyzed by...

Ngày tải lên: 19/02/2014, 05:20

... Similarly, replacing Tyr308 with other aromatic amino acids only slightly affects the inhibition by Zn-ferrocyanide on the enzyme. In contrast, replacing Tyr308 with glycine or serine consider- ably ... Zn-ferrocyanide. Effect of Fld on the inhibition of FNR activities by Zn-ferrocyanide In some photosynthetic systems, such as that of certain algae and cyanobacteria, the FMN...

Ngày tải lên: 19/02/2014, 16:20

... active site of the enzyme by reducing the occupied internal volume, allowing the intro- duction of an extra water molecule [29]. Replacement of the same residue by Ala similarly maintains the same ... manganese. Selectivity of the proteins for their metal cofactor has been demonstrated in vivo [3] and although apoenzymes of each type of SOD may be reconstituted by the addi...

Ngày tải lên: 21/02/2014, 01:21

... CoA in the presence of lucigenin. However, lucigenin itself may increase formation of super- oxide in the presence of enzymes that are capable of catalyzing 1e – reduction of lucigenin directly ... appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the pres...

Ngày tải lên: 21/02/2014, 01:21

... a-tubu- lin. SmGSTO amplification bands were quantified by using GelPro and normalized by comparing to a-tubulin ampli- fication products. Enzyme assays Enzymatic activity towards a range of substrates ... activity was inhibited to different extents by classical GSTs inhibitors. In addition, GSTs’ substrate, CDNB, which inhibits thioredoxin activity, also inhibited GSTO activity. Our p...

Ngày tải lên: 21/02/2014, 01:21