Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

... The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments Youhei Yamagata 1 , Hiroshi ... near the C-terminal region on the opposite side of the N-terminal region on the molecular surface. In the wild- type enzyme, the side chain of Asp266 interacted with t...

Ngày tải lên: 21/02/2014, 03:20

... glycine transfer buffer and assembled into the transfer membrane sandwich against the nitrocellulose membrane. All SDS/PAGE bands in the gel were then transferred onto nitrocellulose membrane ... sequences and full amino acid sequences of the porcine complex II subunits. An abundance of gene sequences of mammalian complex II in the GenBank database was helpful for the...

Ngày tải lên: 19/02/2014, 02:20

... 8A). Next, the levels of Beclin 1 and LC3, two important proteins involved in autophagy, were examined by wes- tern blot analysis in L929 cells treated with oridonin. As shown in Fig. 8B, the level of ... 3-methy- ladenine or small interfering RNA against LC3 and Beclin 1 promoted oridonin-induced cell apoptosis. The effects of p38 and nuclear factor- kappa B in oridonin...

Ngày tải lên: 18/02/2014, 13:20

... pyruvate, and meth- ylamine are bound to the enzyme in this order, and N-methyl-l-alanine and then NADP + are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the same ... substrate and was distinct from alanine dehydrogenase [18] and N-methylalanine dehydrogenase found by Lin & Wagner [17]. Lysine and ornithine were inert as an amine substrate;...

Ngày tải lên: 19/02/2014, 16:20

... run the al- gorithms described in Hu and Liu (2004a) on the same data as the baseline. From Table 2, we can see the precision of base- line system is much lower than its recall. Examin- ing the ... but can be inferred from the text. Qi Su et al. (2008) in- vestigates the problem of extracting those kinds of features. There approach utilizes the associa- tion between...

Ngày tải lên: 20/02/2014, 09:20

... for murine and human applications should provide an important advantage in translation of experimental findings to a clinical setting. Fig. 4. FPGN2-KBP enhances AdV-GFP infection of human DC, ... CD40-binding peptide that has no detectable homology to CD40 ligand (data not shown) and that enhances adenoviral infection of CD40-positive cells of both human and murine origin, inc...

Ngày tải lên: 20/02/2014, 11:20

... easy editing and utilization. Because all of this can be achieved with standard hooks and the IME API of the Microsoft Windows 95 operating system, this tool can be combined with any Windows- ... consulting and the contents of the dictionaries for 'thank you' is displayed. If users double-click on another word in a conversion window or an alternatives window...

Ngày tải lên: 20/02/2014, 18:20

... deficiency in metazoa. Considering that IVD acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the investigation ... cDNA sequences using the RACE method in both strains revealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant,...

Ngày tải lên: 18/02/2014, 04:20

... lipids of the lower leaflet to pack more tightly and extend their acyl chains to maintain the integrity of the membrane. The substantial tilt (and resulting disorder) of the top leaf- let lipids and ... [36,37]. The contraction of the lipid headgroups, and con- comitant disordering of the acyl chains of the lipids in closest contact with Ab, results in no subs...

Ngày tải lên: 18/02/2014, 08:20

... transporters, the BBB endothelium has a receptor-mediated trans- porter system used by proteins, such as insulin, trans- ferrin and leptin, to cross the BBB [13]. Molecular basis of brain angiogenesis and ... VEGF expression and induced angiopoietin-1 (Ang-1), thereby promoting the expression of tight junction proteins and strengthening the bonds between brain endothelial cell...

Ngày tải lên: 18/02/2014, 11:20