Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

... staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The sam- ples were incubated in the dark in the ... sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr and 2D w...

Ngày tải lên: 18/02/2014, 13:20

... of the sole Glyco_18 domain, the C-terminal tail of C. gigas CLPs may not noticeably contribute to the structure and the function of these proteins. Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... cDNA RT-PCR with degenerate primers whose design was based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the...

Ngày tải lên: 19/02/2014, 00:20

... of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part determines ... vivo or in vitro, including characterization of the promoter region of the gene and the associated transcriptional factors. Therefore, delineation of the...

Ngày tải lên: 19/02/2014, 06:20

... enzyme C-terminus, but further enhances the activity of the enzyme whose C-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of ... H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is loca...

Ngày tải lên: 19/02/2014, 07:20

... N-terminal residues of the catalytic domain are under- lined. The preregion is indicated in red, the pro-region in black, catalytic domain in blue and the C-terminal domains in violet. The assumed ... (1988) Nucleotide sequence of the gene for aqualysin I (a ther- mophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced...

Ngày tải lên: 19/02/2014, 07:20

... 30 °C for 10 min. The sample was then boiledfor1minandkeptfrozenat)80 °C for subsequent analysis. To quantitate the amount of glutamate formed, 0.3 mL of the sample was incubated in 1 mL of 50 ... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open reading frame was deleted to result in a frame-shift and appearance of stop codons in the...

Ngày tải lên: 19/02/2014, 12:20

... is presented in Fig. 1. In order to gain further insights into the modes of regulation of TPb, the aim of the current study was to map the minimal transcriptional unit and to identify the key regulatory ... of the previously characterized Prm1, Prm3 belongs to the class of TATA-less promoters [16,25]. In TATA-less promoters, assembly of the pre- initiation co...

Ngày tải lên: 19/02/2014, 16:20

... antibody A in the presence of increasing concentrations of bilirubin. BSP uptake was assayed to track the progress of the antibody-induced inhibition. Figure 5A shows that increasing bilirubin concentrations ... k A and k 0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k 2 and k 1 are the rat...

Ngày tải lên: 20/02/2014, 01:20

... b-strands (1a in Fig. 2), and one large insert of 15 amino acids is present before the first b-sheet of coil 3, which is on the edge of the pronounced sub- strate-binding cleft in EPG2 (Fig. 2). Expression ... that the intrinsic rate of hydrolysis (k int ) in the productive complex is independent of the length of the substrate, K m and V max were used to calcula...

Ngày tải lên: 20/02/2014, 03:20

... localize the ligand binding sites in the leukocyte b 2 integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi- mately 200-amino-acid intervening domain (I domain), which ... anti-ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains. The efficient inhibition of the Fig. 10. Specific binding of purified rec...

Ngày tải lên: 20/02/2014, 11:20