Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... over-expression of Hsp105a seemed to enhance the release of cytochrome c from mitochondria in F9 cells by hydrogen peroxide. Fig. 3. Enhancement of oxidative stress-induced apoptosis by over-expression of Hsp105a. ... induction of apoptosis in F9 cells using SB202190, a potent inhibitor of p38. As shown in Fig. 7A, although hydrogen peroxide-treatmen...

Ngày tải lên: 21/02/2014, 03:20

... occur in the brain include selective loss of dopaminergic neurons in substantia nigra pars compacta and appearance of Lewy bodies consisting of aggregated protein, mainly a-synuclein, in Keywords amyloid; ... results obtained when a-synuclein was incubated in the absence of MPTP. When a-synuclein was incubated alone in the presence of dopamine, it led to inhibition of fib...

Ngày tải lên: 14/02/2014, 19:20

... 1 after deducting basal and background values. Sepsis resulted in a significant increase in the transcription of the mRNA encoding iNOS in heart of iNOS + ⁄ + mice. The expression of iNOS mRNA was ... higher, in iNOS – ⁄ – Fig. 2. Effects of aMT treatment on CLP-induced mRNA levels of iNOS. Ten nanograms of RNA extracted from mouse heart was used, and quantification of i...

Ngày tải lên: 19/02/2014, 00:20

... formation of carbonyl residues in proteins. The enhanced oxidation of pro- teins observed in httEx1-polyQ-expressing cells was not found to correlate with an increased intracellular level of iron. Interestingly, ... httEx1-72Q-EGFP- expressing cells compared to httEx1-25Q-EGFP- expressing cells. The fluorescence index was further increased by about 17% in cells expressing...

Ngày tải lên: 19/02/2014, 06:20

... inhibitors, binding revers- ibly into the active site of the enzyme. Cystatins, including human cystatin C, are general inhibitors of cysteine proteases. For cathepsin B, their K i value is in nanomolar ... nm, depicting a strong interaction between 2A2 mAb and cathepsin B. Determination of the 2A2 mAb binding site on cathepsin B The binding site of 2A2 mAb on cathepsin B was determ...

Ngày tải lên: 18/02/2014, 11:20

... of recombinant HSL. SDS ⁄ PAGE gel displaying the expression and purification of recombinant C-terminally His-tagged rat adipocyte HSL. Lane 1: supernatant fraction of lysed Sf9 cells expressing ... creating high levels of background fluorescence, we investigated the possibility of using lower amounts of ATP for the phosphorylation of HSL. Using radiolabelled ATP, we showed that...

Ngày tải lên: 18/02/2014, 11:20

... observation is in agreement with previous data obtained in the same cells by monitoring apoptosis as the externalization of phosphatidyserine [14]. 1 H NMR ML signals in intact cells and TG signals in lipid ... resulting in normal cell duplication, cell mutation or cell death. Inadequate DNA repair often results in apoptosis. Lack of functional p53 in HeLa cells...

Ngày tải lên: 18/02/2014, 13:20

... MC (2002) Src- induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling. Nat Cell Biol 4, 632– 638. S. Kleiner et al. Loss of E-cadherin function induces uPA FEBS ... of uPA are causally involved in tumor progression, the understanding of these underly- ing intracellular events is of importance. In the present study, we explored the signaling pa...

Ngày tải lên: 19/02/2014, 05:20

... N-terminal domains (N1, N2) of the minor coat protein (g3p) are responsible for binding and infection in E. coli.Thus, incorporation of a library of target proteins between the N-terminal domain ... Protein design by binary patterning of polar and non- polar amino acids. Science 262, 1680–1685. 8. Michnick, S.W. (2001) Exploring protein interactions by inter- action-induced fold...

Ngày tải lên: 19/02/2014, 12:20

... which, in this case, has an inhibitory effect on binding of poly(A) by PNPase. In contrast, an inter- action between Hfq and PNPase would be expected to inhibit both initiation and elongation of ... was proposed to be bound by Hfq at an internal site framed by two stable hairpins [13]. This suggested that Hfq binding is independent of the location of the AU rich region in...

Ngày tải lên: 19/02/2014, 16:20