Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx

... character- ization of the thermally induced aggregation of SK using a variety of techniques. The results provide us with some of the properties of these high molecular mass aggregates and help to delimit the ... corrected using the spectra obtained for solutions in the absence of dye or protein. RESULTS Thermal unfolding of SK under equilibrium The thermal...

Ngày tải lên: 21/02/2014, 03:20

... summarizes recent progress in the understanding of how osmosensing and osmosignaling integrate into the overall context of growth factor signaling and the execution of apoptotic programs. Abbreviations EGF, ... involved in the regulation of metabolism, gene expression and the susceptibility to different kinds of stress [6]. For example, the inhibi- tion of autoph...

Ngày tải lên: 18/02/2014, 16:20

... membrane and mediate interactions with the cytoskeleton and with signaling molecules. On the basis of extensive searches of the human and mouse genomic sequences, it is now known that 18 a-subunits and ... and biological function. For example, only N-glycans located on either the b-propeller of a 5 [55] or the I-like domain of b 1 or b 3 [68] contribute to the re...

Ngày tải lên: 18/02/2014, 17:20

... regulate proliferation and differentiation of human epidermal keratinocytes cultured in vitro [10]. Thus, the vitamin D3 derivatives produced by the action of P450scc are good candidates for use in the therapy of ... proceed with further biological testing of these compounds and the investigation of whether this pathway operates in the human body in vivo. Experimental...

Ngày tải lên: 18/02/2014, 17:20

... traffic and ⁄ or degradation of the IR. How- ever, despite a recent report showing the involvement of Grb10 in the ubiquitination and proteasomal degra- dation of the IR in transfected cells [37], there ... plekstrin homology (PH) and SH2] or phosphorylated insulin receptor-interacting region (PIR) [6] and inhibits the tyrosine kinase activity of the IR [7]. On the...

Ngày tải lên: 18/02/2014, 18:20

... Particular attention is paid to the effects of temperature on enzyme allostery and the influence of temperature in the regulation of PFK activity at both high (euthermic) and low (hibernating) temperatures. Results Selected ... phosphofructokinase. The pH values of the assay mixtures were fixed at 23 °C using 50 m M imidazole–HCl buffer and were then allowed to vary with...

Ngày tải lên: 19/02/2014, 16:20

... How- ever, the matching of the part -of- speech tags of the sentence sequences to the left and right of the in- fix, against the part -of- speech tags of the seed fact, only succeeds for the last three ... acquisition of candidate facts given the extraction patterns, scoring and ranking of the patterns, and scoring and ranking of the can- didate fact...

Ngày tải lên: 20/02/2014, 12:20

... lu219 and aHis245, including the f act that in the ATP synthases of mitochondria and of photosynthetic bacteria the position of these two amino acids in the primary sequence are inverted [24], the ... pronounced in thecaseofthemutant.Thelargedifferencerelativetothe wild-type can be best appreciated by taking the derivative of the fi tting f unctions of the mutant...

Ngày tải lên: 21/02/2014, 03:20

... stefin A and the different enzymes is the average distance between the centre of mass of CA atoms of the stefin molecule and the centre of mass of the CA atoms of the reactive site cysteine and histidine ... A comparison of the structure of the unli- ganded cathepsin B with the structure of the proenzyme, its complexes with chagasin and stefin A sh...

Ngày tải lên: 18/02/2014, 04:20

... change the preference of site utilization; and (c) promotion of the dissociation of Chordin cysteine-rich (CR)-containing fragments from the ligand. They suggest that the first of these three is the ... Smad4). The complexes move into the nucleus and act as regulators of transcription. The activity of BMP is regulated by the binding of extracellular inhibito...

Ngày tải lên: 19/02/2014, 00:20