Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx

... analysis of ligand binding to C-LytA. (A) Con- formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 m M ... intensi- ties of the bicyclic ligands themselves are negligible compared to that of the protein, despite the presence of aromatic moieties, and that the use of an excitatio...

Ngày tải lên: 19/02/2014, 05:20

... reactions of trans-sulfura- tion through the production of homocysteine, biologi- cal methylations of DNA, lipids and proteins, and polyamine biosynthesis through the production of methionine and S-AdoMet ... inactivation of mammalian methionine synthase by nitric oxide. Eur J Clin Invest 26, 167–170. 15 Alston TA (1991) Inhibition of vitamin B12-dependent methionine bio...

Ngày tải lên: 19/02/2014, 05:20

... [51,52]. The O of Ser90 and the S of Cys266 of pea FNR are close to N5 and O4 of the isoalloxazine, which are involved in hydride transfer. The hydroxy group of Ser90 could accept a hydrogen bond and thus ... ld and its apoprotein are able to impede the inhibition of FNR by Zn-ferrocyanide. More remark able, the solely polypeptide interaction between FNR a nd apoFld is...

Ngày tải lên: 19/02/2014, 16:20

... by 1 O 2 -mediated protein peroxides (Eur. J. Biochem. 269) 1925 Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack Philip E. ... protein oxidation; protein peroxides; protein radicals; s inglet oxygen; photo-oxidation. Singlet oxygen (molecular o xyge n in its 1 D g state; 1...

Ngày tải lên: 21/02/2014, 03:20

... by titrating a ligand solution with the proteins. Aliquots of a protein stock solution were added to a ligand solution. The fluorescence of the ligand was measured after each addition of the protein ... °C. The K d values for NP and MP were determined by titrating a protein solution with the ligands. Aliquots of a stock solution of one of the ligands was added to the p...

Ngày tải lên: 19/02/2014, 00:20

... The importance of the L1 loop for DNA binding by RecA ⁄ Rad51 family proteins was observed by mutational and photocrosslinking analyses of RecA [34–37] and a mutational analysis of HsDmc1 [22]. The ... binding by bacterial single stranded DNA-binding protein (SSB) and human replication protein A (RPA) [23,24], we performed mutational analyses on Tyr232 in the L1 loop an...

Ngày tải lên: 19/02/2014, 06:20

... in this model and have shown that the inhibition of MDR1 is a viable potential approach to the reduction of Gb 3 in both the serum and certain tissues of this model. Results MDR1 inhibition in ... Gb 3 levels [9] and the effect of a maintenance dosing of CsA on the recovery of serum Gb 3 levels after termination of ERT was determined. ERT is an effective means of eli...

Ngày tải lên: 19/02/2014, 07:20

... Àðk A =k 0 Þ=½Að1Þ where k A and k 0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k 2 and k 1 are the rate constants of the inhibition of the ... immunization and bleeding of rabbits; to Silvia Zezlina for the affin- ity purification of antibody A from rabbit sera; to Dr Paolo Ermacora and Prof Gior...

Ngày tải lên: 20/02/2014, 01:20

... conformation), and the number of peaks must correspond to the velocity of the catalytic cycle of SERCA. Interestingly, the turnover rate, ATP concentration dependency and TG inhibition of up–down ... 2011) doi:10.1111/j.1742-4658.2011.08222.x Studies of ion pumps, such as ATP synthetase and Ca 2+ -ATPase, have a long history. The crystal structures of several kinds of io...

Ngày tải lên: 14/02/2014, 14:20

... to the inhibition of CYP activity observed in the absence of changes in protein expression in rabbit he- patocytes [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing ... after CLP but were prevented by GdCl 3 . A reduction in the levels of CYP2E1 protein and CYP2B1 and CYP2E1 mRNA expression was prevented by GdCl 3 . Phosphorylation of...

Ngày tải lên: 14/02/2014, 18:20