Tài liệu Báo cáo khoa học: The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli ppt

... The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli Wataru Nishii 1 , Takafumi Maruyama 1 , ... h). RESULTS Preparation of SulA3 ±169 In this study, SulA3 ±169 was obtained from the MBP SulA fusion protein by digestion with factor Xa. The protein was then puri®ed to apparent homogeneity by...

Ngày tải lên: 21/02/2014, 03:20

... differences in the fold of the main chains. None of these includes any of the binding sites, but some are located in the vicinity. Three of the regions with different conformation involve calcium binding ... scaffolds of the inhibitors. Structures of PRK with inhibitors or sub- Fig. 7. Stereo plot illustrating inhibitor binding in subtilases. The binding loop of chymotryp...

Ngày tải lên: 19/02/2014, 07:20

... and auxiliary domains, such as zinc fingers, also involved in nucleic acid binding. No structural studies, how- ever, indicate if these two RNA-binding domains within the same protein in uence each other for RNA ... the RNA-binding affinity by increasing the protein RNA interaction network. In most RRM–RNA complexes, the base stacking on the aromatic residue at RNP 2 positio...

Ngày tải lên: 19/02/2014, 17:20

... contribute to providing chromophore to melanopsin to maintain their light sensitivities. The expression and function of RPE65c in other cell types in the retina remains to be investigated. Earlier ... RPE65c in the inner retina, including Mu ¨ ller cells, but not in the RPE. Furthermore, double-immuno- staining of dissociated retinal cells using antibodies for RPE65c and gluta...

Ngày tải lên: 14/02/2014, 14:20

... they can increase the amount of available phosphate by interacting together. Additionally, fusing PhyH-DI to a single-domain phytase appears to be an efficient way to improve the activity of the ... We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain BPPs would improv...

Ngày tải lên: 14/02/2014, 15:20

... directly involved in ligand binding, presumably abolishing the interaction with signaling partners. The remaining mutations alter amino acids located outside the ligand-binding pocket and reduce protein ... could in uence the infectivity of HIV and also have anti -in ammatory activity. Since 2006, several patients carry- ing a fusion protein, originating from a translocation j...

Ngày tải lên: 14/02/2014, 18:20

... GST alone. The kinase-deficient mutant, OXI1 K45R , in which the lysine residue of the ATP bind- ing domain is mutated to arginine, still interacted with PTI1-4. These data indicate that the kinase ... of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice using recombinant...

Ngày tải lên: 14/02/2014, 19:20

... confirmed by Lin-54 bind- ing to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86]. ChIP experiments on the Cdc2 gene also provided insights into the association of other cell ... proteins with the promoter. E2F4 binding during the cell cycle coincides with the binding of p107 or p130 [61,85]. Another E2F site distal to the E2F ⁄ CDE acts as an acti...

Ngày tải lên: 16/02/2014, 09:20

... HECT domain ubiquitin ligase of the Nedd4 family, characterized by an N-terminal C2 domain responsible for guiding intracellular localization to internal membranes, four WW domains involved in substrate ... proapoptotic protein of the Bcl-2 family that is crucial for death receptor-mediated apoptosis in many cell systems [16,17]. The BH3 domain-only proteins are a subfamily of...

Ngày tải lên: 16/02/2014, 09:20

... determine whether these adja- cent ARE sites played a role in protein binding activ- ity. Initial experiments confirmed that the extended wild-type ARE sequence provided specific protein binding sites ... investigate the means by which these ARE elements cooperate in modulating PAI-2 mRNA stability, REMSA analyses were performed to determine the role of the ARE II and ARE...

Ngày tải lên: 16/02/2014, 09:20