Tài liệu Báo cáo Y học: Dynamic mechanism of nick recognitio

Tài liệu Báo cáo Y học: Dynamic mechanism of nick recognition by DNA ligase ppt

... transition in the DNA ligase catalysis? DYNAMIC MECHANISM OF NICK RECOGNITION BY DNA LIGASE – AHYPOTHESIS According to Doherty et al. [25,34], the adenylylated DNA ligase binds dsDNA forming nonspeciﬁc ... ARTICLE Dynamic mechanism of nick recognition by DNA ligase Alexei V. Cherepanov* and Simon de Vries Kluyver Department of Biotechnology, Delft Uni...

Ngày tải lên : 21/02/2014, 01:21

7
395
0

Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf

... Biochem- istry 40, 6971–6981. 56. Barnes, T.W. III & Turner, D.H. (2001) Long-range cooperativity in molecular recognition of RNA by oligodeoxynucleotides with multiple C5-(1-propynyl) pyrimidines. ... thermodynamic parameters of DNA complexes. Table S2. Direct comparison of temperature-independent and temperature-dependent thermodynamic parameters for DNA complexes Table S3...

Ngày tải lên : 22/02/2014, 07:20

10
447
0

Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf

... 33, 6516–6523. 58 Yang JJ & Van Wart HE (1994) Kinetics of hydrolysis of dansyl peptide substrates by thermolysin: analysis of ﬂuorescence changes and determination of steady-state kinetic ... A. Biochemistry 25, 94–100. 57 Yang JJ, Artis DR & Van Wart HE (1994) Differential effect of halide anions on the hydrolysis of different dansyl substrates by thermolysin. Bioche...

Ngày tải lên : 19/02/2014, 02:20

13
430
0

Tài liệu Báo cáo khoa học: Metabolic control of mitochondrial properties by adenine nucleotide translocator determines palmitoyl-CoA effects Implications for a mechanism linking obesity and type 2 diabetes pdf

... source of fuel for many tissues because they can yield relatively large quantities of ATP, obesity-related persistent oversupply of nonesteriﬁed fatty acids and accumula- tion of triacylglycerols ... diacylglyc- erol] could adversely affect cellular function by direct effects on a variety of enzymes and induction of apoptosis [4]. Tight regulation of intracellular concentratio...

Ngày tải lên : 19/02/2014, 05:20

15
546
0

Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc

... pure enzyme enzyme had a maximum speciﬁc activity of 6 unitsÆ mg )1 [10]. One unit of activity was determined as amount of enzyme catalyzing the decomposition of 1 lmol S-o-nitro- phenyl-L-cysteine ... 2004 Enzymatic a-proton exchange in amino acids (Eur. J. Biochem. 271) 4569 by O-acetylserine sulfhydrylase. By analogy w ith these ﬁndings, a concerted mechanism of isotopic ex...

Ngày tải lên : 19/02/2014, 16:20

7
532
0

Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

... sequences of sugar units was obtained by FAB-MS of the methylated core OS derivative. Figure 2 and Table 3 shows a series of A-type primary glycosyl oxonium ions, and secondary ions, of defined ... LOS, by GLC-MS. The absolute configurations of the sugars mentioned above were deduced by the identification of but-2-yl chiral glycosides in GLC- MS [21]. Sugar linkage analysis (Ta...

Ngày tải lên : 21/02/2014, 01:21

18
718
0

Tài liệu Báo cáo Y học: Identiﬁcation of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

... biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this moiety of ... other hexose dehydratases (data not shown) (Fig. 3). A variety of these enzymes synthesize 4-keto-6-deoxy-GDP- D -mannose, a key intermediate in the biosynthesis of many deoxyhexos...

Ngày tải lên : 21/02/2014, 01:21

9
728
0

Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf

... capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed in vivo and in vitro. DM64 effectively neut- ralized the myotoxic and cytotoxic ... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using...

Ngày tải lên : 21/02/2014, 01:21

11
620
0

Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

... behavior of LMVs composed of dimyristoylglycerophosphocholine (Myr 2 Gro-PCho), dimyristoylglycerophosphoethanolamine (Myr 2 Gro-PEtn) and dimyristoylglycerophosphoglycerol (Myr 2 Gro-PGro) by DSC, ... rmcelhan@gpu.srv.ualberta.ca Abbreviations: GS, gramicidin S; Myr 2 Gro-PCho, dimyristoylglycerophosphocholine; Myr 2 Gro-PEtn, dimyristoylglycerophosphoethanolamine; Myr 2 Gro-PGro, di...

Ngày tải lên : 21/02/2014, 01:21

10
683
0

Tài liệu Báo cáo Y học: A family of expressed antifreeze protein genes from the moth, Choristoneura fumiferana ppt

... stress of a predictable nature such as seasonal d rought, h eat or cold may be endured by entering diapause, a physiological state charac- terized by low m etabolic activity [1]. Many species of ... stringency washes of the CfAFP 337 isoform probe after hybridization. Hybridization and high stringency wash es for probes of isoforms 337 (corresponding t o Afp-Lu1), 10 (to identify...

Ngày tải lên : 21/02/2014, 15:20

9
422
0