Tài liệu Báo cáo Y học: Dynamic mechanism of nick recognition by DNA ligase ppt

... transition in the DNA ligase catalysis? DYNAMIC MECHANISM OF NICK RECOGNITION BY DNA LIGASE – AHYPOTHESIS According to Doherty et al. [25,34], the adenylylated DNA ligase binds dsDNA forming nonspecific ... ARTICLE Dynamic mechanism of nick recognition by DNA ligase Alexei V. Cherepanov* and Simon de Vries Kluyver Department of Biotechnology, Delft Uni...

Ngày tải lên: 21/02/2014, 01:21

... Biochem- istry 40, 6971–6981. 56. Barnes, T.W. III & Turner, D.H. (2001) Long-range cooperativity in molecular recognition of RNA by oligodeoxynucleotides with multiple C5-(1-propynyl) pyrimidines. ... thermodynamic parameters of DNA complexes. Table S2. Direct comparison of temperature-independent and temperature-dependent thermodynamic parameters for DNA complexes Table S3...

Ngày tải lên: 22/02/2014, 07:20

... 33, 6516–6523. 58 Yang JJ & Van Wart HE (1994) Kinetics of hydrolysis of dansyl peptide substrates by thermolysin: analysis of fluorescence changes and determination of steady-state kinetic ... A. Biochemistry 25, 94–100. 57 Yang JJ, Artis DR & Van Wart HE (1994) Differential effect of halide anions on the hydrolysis of different dansyl substrates by thermolysin. Bioche...

Ngày tải lên: 19/02/2014, 02:20

... source of fuel for many tissues because they can yield relatively large quantities of ATP, obesity-related persistent oversupply of nonesterified fatty acids and accumula- tion of triacylglycerols ... diacylglyc- erol] could adversely affect cellular function by direct effects on a variety of enzymes and induction of apoptosis [4]. Tight regulation of intracellular concentratio...

Ngày tải lên: 19/02/2014, 05:20

... pure enzyme enzyme had a maximum specific activity of 6 unitsÆ mg )1 [10]. One unit of activity was determined as amount of enzyme catalyzing the decomposition of 1 lmol S-o-nitro- phenyl-L-cysteine ... 2004 Enzymatic a-proton exchange in amino acids (Eur. J. Biochem. 271) 4569 by O-acetylserine sulfhydrylase. By analogy w ith these findings, a concerted mechanism of isotopic ex...

Ngày tải lên: 19/02/2014, 16:20

... sequences of sugar units was obtained by FAB-MS of the methylated core OS derivative. Figure 2 and Table 3 shows a series of A-type primary glycosyl oxonium ions, and secondary ions, of defined ... LOS, by GLC-MS. The absolute configurations of the sugars mentioned above were deduced by the identification of but-2-yl chiral glycosides in GLC- MS [21]. Sugar linkage analysis (Ta...

Ngày tải lên: 21/02/2014, 01:21

... biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this moiety of ... other hexose dehydratases (data not shown) (Fig. 3). A variety of these enzymes synthesize 4-keto-6-deoxy-GDP- D -mannose, a key intermediate in the biosynthesis of many deoxyhexos...

Ngày tải lên: 21/02/2014, 01:21

... capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed in vivo and in vitro. DM64 effectively neut- ralized the myotoxic and cytotoxic ... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using...

Ngày tải lên: 21/02/2014, 01:21

... behavior of LMVs composed of dimyristoylglycerophosphocholine (Myr 2 Gro-PCho), dimy- ristoylglycerophosphoethanolamine (Myr 2 Gro-PEtn) and dimyristoylglycerophosphoglycerol (Myr 2 Gro-PGro) by DSC, ... rmcelhan@gpu.srv.ualberta.ca Abbreviations: GS, gramicidin S; Myr 2 Gro-PCho, dimyristoylglycerophosphocholine; Myr 2 Gro-PEtn, dimyristoylglycerophosphoethanolamine; Myr 2 Gro-PGro, di...

Ngày tải lên: 21/02/2014, 01:21

... stress of a predictable nature such as seasonal d rought, h eat or cold may be endured by entering diapause, a physiological state charac- terized by low m etabolic activity [1]. Many species of ... stringency washes of the CfAFP 337 isoform probe after hybridization. Hybridization and high stringency wash es for probes of isoforms 337 (corresponding t o Afp-Lu1), 10 (to identify...

Ngày tải lên: 21/02/2014, 15:20