Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc

... (GeneBank accession num- ber AI975843). A 549-bp fragment was amplified by PCR using oligonucleotides GSTX1: 5¢- GTTGTCGACAAA CATCTCAACTAG-3 and GSTX3: 5¢-GTAAGTGTGG GAATAAGATCAAATC from adult S. mansoni ... (para- sitic stage of the intermediate host Biomphalaria glabrata) and adult worms (parasitic stage of human) than in others stages. Expression of SmGSTO was also studied at the...

Ngày tải lên: 21/02/2014, 01:21

... homologues, aqualysin I from Thermus aquaticus and a proteinase from Thermus strain Rt4 1A, the cold-adapted Vibrio -proteinase has a higher content of polar/uncharged amino acids, as well as aspartate ... 5¢-ATGTTAAA GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and 5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG CCG-3¢, cloned into the expression vector pBAD-TOPO, transformed into the E.coliTop10...

Ngày tải lên: 21/02/2014, 01:21

... Technology and Medicine, London SW7 2AZ, UK; 2 Laboratory of Molecular Entomology and Baculovirology, Riken, Wako, Japan Sugar conjugation is a major pathway for the inactivation and excretion of both ... project. A wing disc cDNA library derived from fifth instar B. mori C108 larvae was kindly provided by Dr Kawasaki (University of Utsunomiya, Japan). A total of 1000 clones we...

Ngày tải lên: 22/02/2014, 04:20

... Biochemistry 39, 7131– 7137. 31. Hiraoka, B .Y. , Yamakura, F., Sugio, S. & Nakayama, K. (2000) A change of the metal-specific activity of a cambialistic superoxide dismutase from Porphyromonas gingivalis ... mutagenesis reactions together with oligonucleo- tides ECF-Q69G d(5¢-AACAACGCAGCT GGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAAC CAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTG...

Ngày tải lên: 21/02/2014, 01:21

... phycoerythrocyanin -a8 4-cystein-lyase(PVB- PEC-lyase) from Mastigocladus laminosus, which catalyses both the covalent attachment of phycocyanobilin to PecA, the apo -a- subunit of phycoerythrocyanin, and its isomeri- zation to phycoviolobilin. ... to ENZMES, as an enzyme of the subclass 4.4.1, as an alternative name we proposed holo -a- phycoerythrocyanin synthase, in analogy to the...

Ngày tải lên: 21/02/2014, 03:20

... pattern raises the possibility that Ca 2+ may have a dual mechanism of action in activating PtdEtn-PLD, e.g. Ca 2+ may participate in catalysis as well as facilitate enzyme– substrate interaction. ... preferentially hydrolyses phosphatidyl- ethanolamine (PtdEtn) and phosphatidylserine and does not catalyse a transphosphatidylation with primary short-chain alcohols. We have characterized...

Ngày tải lên: 22/02/2014, 07:20

... cells from human adenocarcinomas Anna Maria Luciani 1 , Sveva Grande 1 , Alessandra Palma 1 , Antonella Rosi 1 , Claudio Giovannini 2 , Orazio Sapora 3 , Vincenza Viti 1 and Laura Guidoni 1 1 Dipartimento ... spectra. Time (day) Time (day) Time (day) Time (day) A/ (Lys + Ala) c A/ (Lys + Ala) i A B A B′ C Fig. 5. Intensity modulation of ML signals from a representative experimen...

Ngày tải lên: 18/02/2014, 13:20

... sequencing chemistry. Real-time quantitative PCR Quantitative RT-PCR analysis was performed using the iCycler apparatus (Bio-Rad, Hercules, CA, USA). Total RNA was isolated from oocytes, embryos, larvae and adult tissues ... (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and antisense primers. Accurate amplifi...

Ngày tải lên: 19/02/2014, 00:20

... Kominato Y, Yamamoto F & Takizawa H (2002) Characterization of the human ABO gene pro- moter in erythroid cell lineage. Vox Sang 82, 39–46. 34 Yasuda T, Awazu S, Sato W, Iida R, Tanaka Y & Kishi ... Kumamoto T, Mor- ikawa N, Masamura K, Tada H, Ito S, Hoshizaki H, Oshima S et al. (2004) Diagnostic use of serum deoxyri- bonuclease I activity as a novel early-phase marker in...

Ngày tải lên: 19/02/2014, 06:20

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... primers: gatggatcccatATGGGTG TTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC annealing just before the putative transmembrane domain (capital lette...

Ngày tải lên: 19/02/2014, 07:20