Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc

... Oxidation of phenols by laccase and laccase- mediator systems Solubility and steric issues Francesca d’Acunzo, Carlo Galli and Bernardo Masci Dipartimento di Chimica and Centro CNR ... was checked by 1 HNMR and HPLC. Spectrophotometric determination of laccase activity The activity of laccase was determined by following the rate of oxidation of 2,2¢-az...

Ngày tải lên: 21/02/2014, 01:21

... Zablotni 1 , Alexander S. Shashkov 2 and Yuriy A. Knirel 2 1 Department of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Poland; 2 N.D. Zelinsky Institute of Organic ... glycosides and the L configuration of lysine by GLC of the acetylated (S)-2-butyl ester. The D configuration of GlcA was established b y a nalysis of 13 C NMR chem...

Ngày tải lên: 21/02/2014, 15:20

... manganese. Selectivity of the proteins for their metal cofactor has been demonstrated in vivo [3] and although apoenzymes of each type of SOD may be reconstituted by the addition of metals, the resulting enzyme ... Woman’s University, Japan) by Professor F. Yamakura (Juntendo University School of Medicine, Japan) and Professor T. Matsumoto (Showa Woman’s University). RESULTS...

Ngày tải lên: 21/02/2014, 01:21

... Enz- ymol. 186, 168–180. 72. Frey, P.A., Flournoy, D.S., Gruys, K. & Yang, Y S. (1989) Intermediates in reductive transacetylation catalyzed by pyruvate dehydrogenase complex. Ann. NY Acad. ... Site-directed mutagenesis of dihy- drolipoamide acetyltransferase and post-translational modifica- tion of its lipoyl domains. In Biochemistry and Physiology of Thiamin Diphosphate Enz...

Ngày tải lên: 21/02/2014, 01:21

... amplification bands were quantified by using GelPro and normalized by comparing to a-tubulin ampli- fication products. Enzyme assays Enzymatic activity towards a range of substrates and inhibitors ... the isomerization of maleylacetoacetate and the regulation of stress kinases and apoptosis [5,6]. Based on their sequence structure, catalytic activitiy, immunogenicity, substrate...

Ngày tải lên: 21/02/2014, 01:21

... Eggertsson 1 and Magnu ´ s M. Kristja ´ nsson 2 1 Institute of Biology, University of Iceland; and 2 Department of Biochemistry, Science Institute, University of Iceland, Reykjavik, Iceland The gene ... intervals and phenyl- methylsulfonyl fluoride was added to a final concentration of 1 m M to inhibit enzyme activity, followed by analysis by SDS/PAGE on 8–25% gels. Table 3....

Ngày tải lên: 21/02/2014, 01:21

... Ventura 1,2 1 Department of Biomedical Sciences, Division of Biochemistry, Laboratory of Cardiovascular Research, 2 Division of Cell Biology, National Institute of Biostructures and Biosystems, and 3 Department of ... directed against human sequences for c-myc and GAPDH and PCR conditions were as previously described [20]. Analysis of c- myc gene transcription by nucle...

Ngày tải lên: 21/02/2014, 01:21

... mechan- isms of PSI reduction by plastocyanin and cytochrome c 6 in the ancient cyanobacteria Pseudoanabaena sp. PCC 6903 and Pro- chlorothrix hollandica.InPhotosynthesis: Mechanisms and Effects (Garab, ... evolution of cyanobacterial and plant plastocyanins. Keywords: cyanobacteria; electron transfer; photosystem I; plastocyanin; weak interaction. Electron-transfer chains like t...

Ngày tải lên: 21/02/2014, 01:21

... ger splicing inhibition by Cl-tetracycline may be explained by the a dditional s ize and hydrophobicity introduced by the substitution, as is often the case in ribozyme inhibition by tetracyclines [33,39,43]. In ... inhibitory effect of erythromycin and Cl-tetracycline were not allevi- ated by Mg 2+ (Fig. 3). Kinetic analyses using increasing concentrations of erythromycin,...

Ngày tải lên: 21/02/2014, 15:20

... method and t he implicit model of interaction can be tested b y additional mutations, by solving the structure of some of the complexes or by other experimental means. CONCLUSIONS We have analysed ... recognize and predict interactions based solely on primary structure and associated physico-chemical properties [27]. In spite of the wealth of approaches presently availab...

Ngày tải lên: 21/02/2014, 15:20