Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... methylation of
hemimethylated DNA in vitro [17,18]. However, Dnmt1
also shows capabilities for de novo methylation of DNA
[15]. Interestingly, the de novo activity of Dnmt1 is
stimulated by binding ... methyl groups are always introduced, one
slowly by Dnmt3a or by de novo methylation catalyzed by
Dnmt1 and the second one very fast. Therefore, this effect
can only double the r...
... depend-
ence of the conversion was determined by varying the
temperature during the incubation from 0.4 to 45 °C.
The pH dependence of the conversion was determined by
varying the pH form 5.66–10.19 (by ... as the standard.
RESULTS
Induction of citral lyase
Initially, the reproducibility of the results was hindered by
variations in the citral lyase activity...
... high
binding affinity of PEP in the presence of Mn
2+
[28]. The
high affinity of PEP in the presence of Mn
2+
suggests a
specific interaction between these two ligands in the enzyme
active site. In the ... structure of E. coli PEP carb-
oxykinase (1OEN) is covalently linked to the carbonyl carbon of the
P-pyridoxyl group (PL) through an imino linkage. The gree...
... were
recently introduced for the preparation of C-6 aldehyde
derivatives of Glc [15]. The key step is the use of Collins
reagent for the selective oxidation of the primary trimethyl-
silyl ether of the ... that
dephosphorylation and/or catalytic turnover of EII
Glc
,
rather than binding of Glc, enhanced the reactivity of
Cys421. As Cys421 is the only invariant...
... chains of pectin. This is in agreement with a
previous study in which the synergy of enzymes degrading
the pectin side chains was studied [34]. GALA had a positive
effect on the activity of the ... with both the pectinolytic genes encoding main
chain cleaving enzymes as well as with the arabinanolytic
genes [1]. The absence of expression of galA in the presence...
... of
Mn[G77Q] and the very low thermostability of Fe[A141Q]
(Fig. 5). Each of these mutants has two glutamine residues
in their active sites; one from the Ônaturally occurringÕ
residue, the other engineered ... residues, causing a shift in
the position of Y3 4 and a twist in the ring of the metal-
binding H81. Thermostability is not a new phenomenon
amongst SODs; th...
... characterization of specific enzymes that
are involved in the biosynthesis of these structures and may
lead to the discovery of potential therapeutic targets. In
addition, the correlation of carbohydrate ... conclusively demonstrate the linkage of the
capsule polysaccharide to its membrane anchor and are
currently trying to determine the nature of the membrane
lin...
... previously [20]. The serine, threonine and tyrosine
contents of the hydrolysates were corrected for destruction
during the hydrolysis by extrapolation to zero time hydro-
lysis. The amino acid ... revealed the
predominance of two amino acids: alanine (39.2%) and
glycine (30%). Interestingly, the proportions are very similar
to those found in WSM for alanine and glycine.
Curi...
... appearance of the ThDP adduct with the substrate
moiety during the complex-catalyzed succinyl-CoA hydro-
lysis [72] accounts for the inactivation by dihydrolipoamide
in the presence of succinyl-CoA ... (b) the
thiyl radical of the lipoyl residue should efficiently interact
with the E1 catalytic intermediate, as the lipoyl-bearing
domain of E2 is designed for this in...