Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx
... allow chemical characterization of each. CO3-1 was identified as trehalose by TLC as well as by MALDI-TOF MS analysis of the products obtained by permethylation and hydrolysis, andalsoby 1 H-NMR. Oligosaccharide ... concentration of 70% to precipitate glycogen and any other trichloroacetate-soluble polymers present in the cytoplasm. The alcohol mixture was allowed to stand over...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... catalyzed the ring fission between C2 and C3 of 4-amino-3-hydroxybenzoic acid, with the consumption of 1 mol of O 2 per mol of substrate. The enzyme was therefore designated as 4-amino-3-hydroxy- benzoate ... amidol, 3-hydroxyanthralinic acid, 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshly prepared and used immediately. Effect of various c...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxidation of the primary trimethyl- silyl ether of the ... against inactivation. To the contrary, the presence of Glc slightly sensitized EII Glc for inactivation by the isothiocyanates 1e and 3d. On the other...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis ... chains of pectin. This is in agreement with a previous study in which the synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positive effect o...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... governed by one or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues. Further analysis of these and other mutant SODs is currently underway. ACKNOWLEDGEMENTS We ... in a hydrogen-bonding network involving other residues and solvent molecules. An unex- pected result was the extremely high thermostability of Mn[G77Q] an...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate ... A.M., Wu, Y. , Young, N.M. & Wakarchuk, W.W. (2000) Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase g...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... composition was very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy- lysine and phosphoserine were not detected in WSM, WIM, EDTA-SM and EDTA-IM. The comparison of the amino ... Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phase HPLC of their phenylisothiocarbamate derivatives [26], as reported previously [20]. Th...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... mechanisms of the inactivation of the complex by the thiyl radical of dihydrolipoate, we took into account that (a) the overall activity (Fig. 2) is decreased du...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... decades of life and severely impair visual acuity in adulthood. Their therapy is restricted to keratopl- asty and phototherapeutic keratectomy by Excimer laser. Unfortunately, the benefits of these therapies ... in a phenotypic variant of granular corneal dystrophy type 1 (GCD1), Arg124His (R124H) [1] in granular corneal dystrophy type 2 (GCD2, Avellino dystrophy) a mixed type of...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc
... purification of oligodeoxynucleotides used in the binding studies Oligonucleotide synthesis was performed on an Applied Biosystems 394 DNA synthesiser using cyanoethyl phos- phoramidite chemistry. The ... Also, E. coli RuvA has the ability to bind to the duplex arms of the Holliday junctions after specifically binding to the Holliday junction at the crossover point. From...
Ngày tải lên: 21/02/2014, 01:21