Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... better clarify the occurrence of LOXs and their influence on hazelnut seed quality, we carried out the biochemical and molecular characterization of the main LOX isoforms expressed during seed development. ... Characterization of hazelnut seed lipoxygenases (Eur. J. Biochem. 270) 4375 Biochemical and molecular characterization of hazelnut ( Corylus ave...

Ngày tải lên: 21/02/2014, 00:20

... standard molecular weight proteins. The flow rate was set to 0.8 mL Æmin )1 . The subunit molecular mass was also estimated by SDS–PAGE. Characterization and comparative analyses of HYD Js and ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate...

Ngày tải lên: 18/02/2014, 08:20

... members of the MAPEG superfamily a set of representative members were selected as seeds. The seeds were the human member proteins of MGST1-3 (Uni- prot-Swissprot identifiers P10620, Q99735 and O14880); FLAP ... DNA and RNA were performed as described previously [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the co...

Ngày tải lên: 19/02/2014, 17:20

... localization and association with the development of fruiting bodies. J. Gen. Microbiol. 125, 147–157. 328 S. Chen et al. (Eur. J. Biochem. 271) Ó FEBS 2003 Biochemical and molecular characterization of ... K m value of 0.03 m M and a V max of 16.4ÆUmgprotein )1 . Corresponding values for syring- aldazine and 2,6-DMP under optimal conditions were 0.01 m M and 4.9 UÆmg...

Ngày tải lên: 07/03/2014, 15:20

... ª 2009 FEBS Biochemical and molecular characterization of purified chicken pancreatic phospholipase A 2 Aida Karray, Fakher Frikha, Abir Ben Bacha, Yassine Ben Ali, Youssef Gargouri and Sofiane ... non- glycosylated monomeric protein with a molecular mass of 14 kDa and a specific activity of 400 UÆmg )1 in the presence of 1 mm sodium taur- odeoxycholate and 4 mm CaCl 2 wi...

Ngày tải lên: 30/03/2014, 01:20

... Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, University of Wisconsin–Madison, USA Introduction Upon ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and c...

Ngày tải lên: 14/02/2014, 22:20

... structure of cN-II showing interfaces A and B and the Mg 2+ site. The inset shows the tertiary structure of each subunit. Effector sites 1 and 2 and the active site are shown. Table 2. Effect of point ... transfer of phosphate esterified in the 5¢ position of 6-hydroxypu- rine monophosphate nucleosides [1]. The transfer of phosphate can lead to phosphorylation of inosine,...

Ngày tải lên: 15/02/2014, 01:20

... four times. (A) PRTFDC1 and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined ... activity [10]. The structural characterization of numerous com- plexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17]. The st...

Ngày tải lên: 15/02/2014, 01:20

... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational change rates versus rates of FMNH 2 formation ... Model and simulations of cytochrome c reduction by NOS enzymes. (A) Scheme of cytochrome c reduction. The model uses four kinetic rates: dissociation (k 1 ) and association (...

Ngày tải lên: 18/02/2014, 11:20

... & Buckmann AF (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo- specificity of 7-alpha-hydroxysteroid and 12-alpha-hy- droxysteroid dehydrogenase. ... isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. Biochem...

Ngày tải lên: 18/02/2014, 11:20