Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... 505–524. Academic Press, New York, USA. Ó FEBS 2003 Characterization of ccNiR subunits (Eur. J. Biochem. 270) 3915 The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and ... periplasmic pentahemic cytochrome c, NrfB [22]. These important developments demand the re-examina- tion of the biochemical properties of D. d...

Ngày tải lên: 21/02/2014, 00:20

... 168PhyA sig- nificantly enhanced their catalytic efficiencies. This is the first report to elu- cidate the substrate specificity of the incomplete domain and the functional relationship of tandemly repeated ... to increase the catalytic efficiency of PhyH-DII. Further- more, the catalytic efficiency of PhyH was much greater than that of PhyH-DI and PhyH-DII combined (5.73...

Ngày tải lên: 14/02/2014, 15:20

... indicated in bold, were used according to the proce- dures of the QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA): C1 53S-up, 5¢-TGCATC TTGCACTACCAACTCTCTTGCTCCCTTTGTC-3¢; ... forward (NcoI site underlined), 5¢-TGTGA CCATGGCCAAGC-3¢; reverse (BamHI site underlined), 5¢-CAA GGATCCCTCA CTTC-3¢. The PCR product was digested by NcoI and BamHI restriction enzym...

Ngày tải lên: 19/02/2014, 05:20

... grapheme detectors as a function of frequency of occurrence and differences in the strength of the connections between graphemes and phonemes as a function of association probability would then explain ... was a central theoretical assumption of these models compared to connectionist ones. As the DRC model is largely based on the interactive activation principles...

Ngày tải lên: 20/02/2014, 19:20

... but was scarcely degraded in t he absence ATP during 120 min of incubation. On the other hand, no degradation of MBP was observed either in the presence or in the absence of ATP under the conditions ... by dialysis. The CD spectrum of SulA3±169 showed that the protein had a signi®cant amount of secondary structures, and the secondary structure contents were almost id...

Ngày tải lên: 21/02/2014, 03:20

... forward, 5¢-ACCCCAGCTCAGGGTTTAGGCTTCT-3¢; NFKBIA reverse, 5¢-TGGCTGGGGATTTCTCTGGG-3¢; OPTN forward, 5¢-ACCCGGGTCCCAGCCTCGAC-3¢; OPTN reverse, 5¢-GACAGCCAGCCGCTCCCTGC-3¢; SPI-B for- ward, 5¢-TCCAGCTCCTGTCCCATCTC-3 ... SPI-Brv (5¢-ACTGAAGCTTGGTGGTGCCGGGCGGGCTGT-3¢), and the SacI ⁄ HindIII tailed primers CASP4fw (5¢-ACT ACGCGTAGCAAAGAGTGCTGCCTCCTCCTTCCT-3¢) and CASP4rv (5¢-ACTCTCGAGTTCCCTG...

Ngày tải lên: 18/02/2014, 18:20

... addresses Cardiff School of Biosciences, Biomedical Sciences Building, Cardiff, UK àSICIT 2000 S.p.A., Chiampo, Italy §Syngenta Seeds, Enkhuizen, the Netherlands –UMR Ecophysiology and Grape Functional Genomics, ... However, the AGC VIII kinases represent a plant-speci c sub- family characterized by a conserved DFD amino acid motif in subdomain VII of the catalytic domain and...

Ngày tải lên: 14/02/2014, 19:20

... include control through p53. Cell cycle arrest can be medi- ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc2 5C. CDE ⁄ CHR-dependent ... another report confirmed the CDE in the Cdc2 promoter as being dif- ferentially occupied by protein complexes during the cell cycle [9]. Mutation of nucleotides close to the C...

Ngày tải lên: 16/02/2014, 09:20

... apoptosis. The mitochondrial release of cytochrome c and second mitochondria-derived activator of caspase (Smac ⁄ DIABLO) allows for the formation of the apoptosome, a complex that enables the activation ... produced (Fig. 1D, lane 2). In the presence of lactacystin, a sig- nificant increase in the amount of tBid–GFP present in the extract, both in control conditi...

Ngày tải lên: 16/02/2014, 09:20

... AGTAAGGAAAATA AGCTTGGGCATGATCCC Reverse (nt 1520–1491 PAI-2) SJS174 GCTCACTGCCTA AGCTTTGTAGCTAATAAAG Forward (nt 1596–1625 PAI-2) SJS175 CTTTATTAGCTACA AAGCTTAGGCAGTGAGC Reverse (nt 1625–1596 PAI-2) SJS259 CTTTGTTATTTATTAT GCATTCCTATGGTGAGTT ... AACTCACCAT AGGAATGCATAAGCTTTAACAAAG Reverse (nt 1585–1552 PAI-2) SJS167 CCTCTTACACTTGCTTTTGAC Forward (nt 455–474 pTETBBB) SJS170 GCAAAGGTGCCTTTGAGGT...

Ngày tải lên: 16/02/2014, 09:20