... supernatant and the membrane frac-tions was similar to those of GGN, GGD, GGS, andAAG (Figs 2B and 4B). Interestingly, similar to thecase of GGN mutant, a small but significant amount of proteins ... stroma and to the membrane (Fig. 4A, lanes 21–24; Fig. 4B,C) in a waysimilar to GGA mutant (Fig. 4A, lanes 9–12;Fig. 4B,C). Import of GGE mutant was less efficientthan that of other proteins, ... extended the analysis of the poly-glycine stretch of the Toc75 transit peptide in order tobetter understand the targeting mechanism of thechloroplast outer envelope membrane protein. We divi-ded...