Tài liệu Báo cáo khoa học: Membrane orientation of laminin binding protein An extracellular matrix bridging molecule of Leishmania donovani pdf

... Membrane orientation of laminin binding protein An extracellular matrix bridging molecule of Leishmania donovani Keya Bandyopadhyay* , †, Sudipan Karmakar*, Aruna Biswas and Pijush ... either anti-LBP IgG or anti -laminin IgG. Proteolytic digestion of L. donovani membrane L. donovani membrane preparations were made according to the method described previ...

Ngày tải lên: 21/02/2014, 00:20

... the importance of anchoring the tail of the ecto- domain for both IGF-II and Man-6-P ligand binding. Receptor binding on resin resembles a patchwork model, where a multivalent ligand can cross-bridge between ... (1986) Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding proteins and competi- tiv...

Ngày tải lên: 18/02/2014, 08:20

... pool of translocator proteins is released rapidly and concurrently, and effectors are translocated in an ordered manner [16,17]. Translocon proteins presumably travel through the interior of the ... formation The composition of the host cell membrane appears to be a critical point for the invasion of bacteria, insertion of translocators and functionality of the pore. Target m...

Ngày tải lên: 14/02/2014, 22:20

... supernatant and the membrane frac- tions was similar to those of GGN, GGD, GGS, and AAG (Figs 2B and 4B). Interestingly, similar to the case of GGN mutant, a small but significant amount of proteins ... stroma and to the membrane (Fig. 4A, lanes 21–24; Fig. 4B,C) in a way similar to GGA mutant (Fig. 4A, lanes 9–12; Fig. 4B,C). Import of GGE mutant was less efficient than that of o...

Ngày tải lên: 19/02/2014, 07:20

... m t1 ÞÁD ð9Þ where lnk f1 and lnk f2 are folding constants associated with first and second transitions, respectively, at any given dena- turant concentration, and lnk u1 and lnk u2 are the unfolding J. ... (1991) Denatured states of proteins. Annu Rev Biochem 60, 795–825. 36 Matouschek A & Fersht AR (1993) Application of physical organic chemistry to engineered mutants of proteins:...

Ngày tải lên: 18/02/2014, 13:20

... 4407 direct substrate of the reductase and can be progres- sively regenerated from the a anomer by spontaneous anomerisation. Kinetic analysis of the recombinant proteins from C. glabrata and A. aeolicus ... RIB7 protein of Methanocaldococcus jannaschii (accession number Q58085); ARCFU, RIB7 protein of Archaeoglobus fulgidus DSM 4304 (acces- sion number O28272); CANGL (CglRED), RI...

Ngày tải lên: 18/02/2014, 18:20

... mass lynx v.4.0 software package. Acknowledgements We thank NSERC of Canada for financial support (M. J. Stillman) and Postgraduate Scholarship (K. E. Rigby Duncan). We also thank Professor R. J. ... noncooperative metal binding to the a domain of human metallothionein Kelly E. Rigby Duncan and Martin J. Stillman Department of Chemistry, The University of Western Ontario, London, ON,...

Ngày tải lên: 19/02/2014, 00:20

... buffer. Shown are stefin A and stefin B at pH 5 and variant 2 of ste- fin B and the P79S mutant of the variant at both pH 7 and pH 5. Each panel is a plot of heat change on ligand addition (kcalÆmole )1 ) against ... the ligand-macromolecule reaction occured. A binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macro...

Ngày tải lên: 19/02/2014, 06:20

... considering membrane components, the secretory (Sec) translocon is known to play an important role as a protein- conducting channel for membrane integra- tion and translocation [33]. The majority of membrane proteins ... mature protein. Synthesis of membrane proteins for minimal cells The goal of the new and rapidly developing field of synthetic biology is the development...

Ngày tải lên: 19/02/2014, 06:20

... relevant to the regulation of AC proteins. In view of the important and antagonistic effects of ADF ⁄ cofilin and phosphate on actin dynamics, we examined here the binding of cofilin to F-actin and ... quenchers. These changes transform the nucleotide cleft of F-actin to G-actin-like. Pi regulation of cofilin binding and the cofilin regulation of Pi binding to F-actin can be i...

Ngày tải lên: 19/02/2014, 07:20