Tài liệu Báo cáo khoa học: Glucose sensing in the intestinal epithelium pdf

... cultured in medium containing 5 m M glucose, transfected with the ovine SGLT1 promoter fragment, and then either maintained in the same medium or transferred to one containing 25 m M glucose, in the ... sensing (Eur. J. Biochem. 270) 3383 that there is an increase in intracellular cAMP levels in response to increasing levels of medium D -glucose. Involvement of a GPCR...

Ngày tải lên: 21/02/2014, 00:20

... recovered, only the longest amino acid sequences that included the DNA binding, hinge and ligand-binding domains were used for this analysis, except for the Knirps fam- ily members that lack the LBD. In D. ... reorganization of the follicular epithelium in the resulting adults [40]. DmE75A and DmE75B have been implicated in defining the transition stages 8 and 9 of the...

Ngày tải lên: 18/02/2014, 13:20

... interpretation in distinguish- ing interacting from noninteracting proteins. One Fig. 1. Schematics of library designs. The helical wheel diagram looks down the axis from the N-terminus to the C-terminus. ... focused on small molecule inhibitors, but the potential of using peptides as the starting point in the generation of therapeutics is now a growing area [18,19]. Peptide...

Ngày tải lên: 18/02/2014, 13:20

... suggesting their complete cellular segregation from A-type lamins when the NE is disas- sembled [10a]. In mammalian cells lamins bind in vitro to many known INM proteins, including emerin, MAN1, ... complexes by the NE proteins as illustrated in Fig. 1. By serving as docking sites at the INM, LAP2b, LBR, and poss- ibly other INM proteins, can anchor DNA, chromatin and chromatin mo...

Ngày tải lên: 19/02/2014, 02:20

... lead to the complete mineraliza- tion of the pyrrolidine ring of nicotine, which after oxidation by 6-hydroxy-l-nicotine oxidase, is cleaved off from the pyridine ring of nicotine in the form ... enzymes involved in nicotine catabolism will have applications not only in the bioremediation of nicotine waste, but also in the supply of nicotine derivatives as starting materia...

Ngày tải lên: 19/02/2014, 07:20

... computing the G + C content in sliding windows moving along the genome. Fig. 4. The negative cumulative GC profile for GGA12 marked with the segmentation points obtained. The bottom five plots show the ... all the seg- ments obtained at t 0 ¼ 100 without the constraint of the minimum length in the draft genome sequence of chicken. (A) The length dis- tribution of all...

Ngày tải lên: 19/02/2014, 07:20

... in epileptic patients [27]. Other mechanisms may be associated with the increase in ROS levels in the epilepsy model as well as in neurodegeneration observed in epileptic humans [18,28]. During ... findings show that pilocarpine induces SE, which can produce alterations in superoxide dismutase and catalase activities in different areas, thereby pro- tecting the brain from n...

Ngày tải lên: 19/02/2014, 16:20

... productive binding of steroid substrates: in the first, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive conformation. ... unusual in having two histidine residues (rather than the His-Asn pair seen in OYE, MR and some of the other member proteins; Fig. 1B) in the acti...

Ngày tải lên: 20/02/2014, 02:21

... lCiÆmL )1 [ 3 H]inositol for 24 h in inositol-free medium containing 10% (v ⁄ v) dialyzed fetal bovine serum. Cells were then washed and incubated in NaCl ⁄ P i containing 20 mm Li + with the agonist ... RGS domain in the amino-terminus of G protein-coupled receptor kinase 2 inhibits Gq-mediated signaling. Int J Mol Med 5, 335–340. 20 Chen CA & Manning DR (2001) Regulation of G...

Ngày tải lên: 20/02/2014, 03:20

... determined by incubating the diluted recombin- ant proteins in the standard reaction mixture in the presence of increasing concentrations of PP i (0.05–0.5 m M )at55°C. Data were fit to the appropriate ... rate was then calculated. Larval molting inhibition assay To confirm whether the native PPase enzyme is involved in the molting process, we examined the effects of two PPa...

Ngày tải lên: 20/02/2014, 11:20