Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... 2003 Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner Øyvind Dahle 1 , Tor Ø. Andersen 1 , Oddmund Nordga ˚ rd 1 , ... of sumoylation was greatly enhanced by cotransfection of PIASy, an E3 ligase for SUMO-1. The functional consequence of abolishing sumoyla...

Ngày tải lên: 21/02/2014, 00:20

... Rosano C, Weisel JW, Horita DA & Hantgan RR (2008) Integrin conformational regulation: uncoupling extension ⁄ tail separation from changes in the head region by a multiresolution approach. Structure ... 166, 5629–5637. 44 Zang Q & Springer TA (2001) Amino acid residues in the PSI domain and cysteine-rich repeats of the integrin b2 subunit that restrain activation of the in...

Ngày tải lên: 18/02/2014, 04:20

... experiments and theoretical analyses suggest that membrane association and plasma membrane targeting of K-Ras are a conse- quence of the electronegative sensing function of the C-terminal domain of this ... rather than alteration of sur- face potential. In summary, our studies indicate that composition of the outer leaflet affects membrane localization of K-Ras, and ar...

Ngày tải lên: 19/02/2014, 00:20

... Production of human sali- vary type cysteine proteinase inhibitors (cystatins) by an Escherichia coli system and partial characterization of recombinant cystatin S and its mutant (117 arginine fi tryptophan). ... pro- tein inhibitors act as one of the main means of regulation [8]. The best characterized are the cystatins, which comprise a superfamily of evolutionarily related pro...

Ngày tải lên: 19/02/2014, 17:20

... Interestingly, volvatoxin A1 contains 393 amino acid residues that closely resemble a tandem repeat of volvatoxin A2 . Volvatoxin A1 contains two pairs of amphipathic a- heli- ces but it lacks a heparin-binding ... demonstrated that volvatoxin A1 and volvatoxin A2 may form a complex. Our results suggest that this occurs via the interaction of one molecule of volvat...

Ngày tải lên: 19/02/2014, 06:20

... response to acti- vation of AMPK cannot lead to production of ATP because of lack of mitochondrial ADP. As AMPK sti- mulates cellular fatty acid uptake [29] and the availab- ility of circulating ... extramitochondrial AMP formation experimentally, as we used P 1 ,P 5 -di(adenosine-5¢)-pentaphosphate (Ap 5A) as inhibitor of adenylate kinase to prevent depletion of available ATP a...

Ngày tải lên: 19/02/2014, 05:20

... 47-kDa protein monomer. GO cata- lyzes the dioxygen -dependent oxidative deamination of primary and secondary amines (sarcosine, N-ethylgly- cine, and glycine) and d-amino acids (d-alanine and ... gene-shuffling approach to molecular evolu- tion, and a rational, structure-based approach to protein engineering. In addition, we offer a rationale for the means by which the modifications m...

Ngày tải lên: 14/02/2014, 14:20

... an increase in the amount of DRM-associated tau isolated from wild-type neurons (Fig. 5A) . In contrast, pervanadate did not appear to induce any change in the amount of tau in the DRM fraction ... plays a dominant role in mediating the association of tau with Fyn-SH2. Tau phosphorylated at Tyr18 has been detected in a proportion of tangles in early AD brain [31], an...

Ngày tải lên: 14/02/2014, 14:20

... these asco-laccases at high protein concentrations. Database Structural data are available in the Protein Data Bank database under the accession numbers 3PPS and 2VDZ Structured digital abstract l laccase ... Electron Corporation, Waltham, MA, USA). The reactions were started by addition of substrate, and the rate of substrate oxidation was measured by monitoring the change in absor...

Ngày tải lên: 14/02/2014, 18:20

... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma- tional rearrangement that exposes the NLS in an extended conformation. Database Structural data are available in the ... Characteristics of the importin -a: CLIC4 NLS peptide interaction. NLS residue Interactions a Van der Waals’ contact area (A ˚ 2 ) b Buried surface area (A ˚ 2 ) RSCC c Main c...

Ngày tải lên: 14/02/2014, 19:20