Tài liệu Báo cáo khoa học: Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS docx

... fragments in E. coli mutants grown on 20% [U- 13 C] and 80% unlabeled glucose. Ó FEBS 2003 Metabolic flux profiling in E. coli (Eur. J. Biochem. 270) 891 Metabolic flux profiling of Escherichia coli mutants ... conducted in shake flasks. Metabolic flux ratio analysis of E. coli mutants of central metabolism The above developed metabolic flux...

Ngày tải lên: 20/02/2014, 23:20

... study Unfolding of the hexameric Hfq protein in urea was monitored with a PTI A1010 fluorimeter using the intrinsic tyrosine fluorescence of Hfq. Protein concentration was 0.1 mgÆmL ) 1 . Tyrosine residues ... center-left), indicating the presence of a stain-excluding region, which can be assigned to the C-terminal domain not present in the proteolysed protein. This domain is at the...

Ngày tải lên: 19/02/2014, 12:20

... based on the concept of glycan profiling, and utilizes lectins, a group of glycan-discriminating proteins. In general, however, the glycan–lectin interaction is relatively weak in comparison with, ... analysis of glycans. In general, the current principal technologies in use are: (a) de novo sequencing; (b) glycan mass fingerprinting [29]; and (c) MS n spectral matching [2,30–32]...

Ngày tải lên: 16/02/2014, 08:20

... HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of bone marrow origin, including KG1 myeloid cells, ... [3–5]. Characteristically, human HO-1 contains no cysteine residue [6], whereas HO-2 contains at least two copies of a potential heme-binding site, consisting of the cysteine and p...

Ngày tải lên: 19/02/2014, 06:20

... mechanisms underlying increased cardioprotection in younger female mice are unclear. We hypothesized that serine-threonine protein kinase (protein kinase B; Akt) triggers a metabolic gene switch ... substrate. In agreement, lower PDK-4 gene expression in female hearts indicates that myocardial glucose metabolism may be increased in parallel. As optimization of glucose metabolis...

Ngày tải lên: 18/02/2014, 16:20

... 68% of the decrease in J o , 68% of the decrease in J p , 56% of the increase in the QH 2 ⁄ Q ratio, 70% of the increase in Dw, 72% of the increase in the ATP in ⁄ ADP in ratio, and 59% of the ... contributed 75% of the reduction in J o , 76% of the reduction in J p , 68% of the increase in Dw, 88% of the increase in the ATP in ⁄ ADP in ratio, and...

Ngày tải lên: 19/02/2014, 05:20

... gain -of- function mutants of PCSK9 reduced binding of [ 125 I]TC-PCSK9. The Scatchard plot of the binding-inhibition curve was curvilinear, indicative of high-affinity and low-affinity sites for PCSK9 binding ... [ 125 I]TC-PCSK9 binding to HepG2 cells (data not shown), further supporting the specificity of the binding. Estimation of binding affinity of wild-type and two mutant v...

Ngày tải lên: 14/02/2014, 14:20

... isolation Using phytase screening and low phosphate media, three strains with phytase activity were isolated from the alpine tundra soil of China No. 1 Glacier in Xinji- ang, China. Strain HJB17 ... for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China Keywords dual domain; fusion protein; phytate;...

Ngày tải lên: 14/02/2014, 15:20

... participating Table 1. Amino acid frequencies (%) in a-amylase linkers, in a set of globular proteins, in the Swiss-Prot databank and in intrinsically unstructured proteins. Amino acid Linkers a Globular proteins b Swiss-Prot c Intrinsically unstructured proteins b Ala ... the ring may sterically disfavor extensive bending in this region, which could result in unwanted interacti...

Ngày tải lên: 14/02/2014, 18:20

... uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrates. In UpG, the binding of the guanine moiety is deterred by exocyclic O 6 . Close inspection ... of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea on the behavior of s...

Ngày tải lên: 14/02/2014, 22:20