... into the expression vec-tor pET-15b to make pATF-20, and the final constructwas transformed into E. coli Tuner (DE3). The recombi-nant McoP was purified to homogeneity by using metalaffinity and ... 21016–21022.35 Durao P, Chen Z, Fernandes AT, Hildebrandt P,Murgida DH, Todorovic S, Pereira MM, Melo EP &Martins LO (2008) Copper incorporation into recombi-nant CotA laccase from Bacillus subtilis: ... nitrate to dinitrogen gas is accom-plished by different types of metalloenzymes in foursteps: nitrate to nitrite, nitrite to nitric oxide, nitricoxide to nitrous oxide, and finally nitrous oxide to...