... KCl and anapproximately twofold decrease in binding at 0 and 500 mm KCl [32]. Thus, to address the in uence of thereaction conditions on the binding of Fpg and OGG1 to damaged DNA, we determined ... composition of the reaction mixture. To minimizethe impact of protein binding to non-damaged DNA,shortened 12-mer ligands were used, identical in sequence to the central part of the 23-mers used in the ... Fpg and OGG1, and the kinetic parameters deter-mined in the absence of polyamines and crowdingagents need not be corrected when considering the intra-cellular environment. Biotin and caffeine...