... that conephotoreceptors recovered light sensitivity followingphotobleaching when the cone photoreceptors are con-nected with other retinal cells, but not with the RPE;rod photoreceptors did not ... °C for 5 min fol-lowed by 35 cycles of 94 °C for 1 min, 58 °C for 1 min and72 °C for 2 min. After agarose gel electrophoresis, the PCRproduct was purified and cloned into the pGEM-T Easyvector ... USA) with an oligo-dT primer and random hexamer. PCR was per-formed with PCR master mix (Roche, Indianapolis, IN,USA) at 94 °C for 5 min followed by 35 cycles of 94 °C for 30 s, 48 °C for 30...