Tài liệu Báo cáo khoa học: "Different Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human Assessors" docx

... Association for Computational LinguisticsDifferent Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human AssessorsHoa Trang DangInformation Access ... “information nuggets” to assess an-swers to complex questions. As it has become thede facto standard for evaluating such systems, theresearch community stands to beneﬁt from a betterunderstanding ... se-ries and run as factors, and the binary F-score asthe dependent variable. We found signiﬁcant differ-ences between series and between runs (p essentiallyequal to 0 for both factors). To determine...

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... added to the C-terminal end of the13-amino-acid peptide corresponding to the primarysequence of the Tat protein to provide a sulfhydryl group for further ligation to a ¯uorochrome or to a cargomolecule. ... NaCl/Pi(pH 7 .3) and ®xed in3.7% (v/v) formaldehyde in NaCl/Pi for 5 min at roomtemperature. For experiments at 4 °C, the protocol wasthe same except that all incubations were performed at4 °C ... and biological evidence indicates that the cellular uptake of theTat basic peptide does not involve binding to HS proteo-glycans and endocytosis.EXPERIMENTAL PROCEDURESPeptide synthesis and...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... ACT1 and ACT2 from differentchains associate side-by-side to form an eight-stranded b-sheet, and two eight-stranded b-sheets faceeach other perpendicularly. In CgAKb, both eight-stranded ... we describe the crystal structures ofTtAKb in two forms, Thr-bound and Thr-free, and discuss the regulatory mechanism of Thr and the struc-tural features responsible for the high thermostabilityof ... (Asp26-Od2 for theamino group), hydrogen bonds (Gln50-Oe1 and Ile126*-O for the side chain hydroxyl group; Asn125*-Od1 and Ile126*-O for the amino group; Ile30-N,Ile126-N and Asn125*-Od1 for the...

Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf

... cofactor domains of the OaPDH and TbPDH structures [13]. The superposition of sub-unit A of complex I onto subunits A, B, C of complex II and III yield rmsd-values of 0.8 A˚ for subunit A and 1.2 ... content to Fig. 1. (A) Catalytic reaction of PDH and two intermediate states. (B) Structures and numbering of the inhibitors PEX (two resonance forms) and PEA.6-Phosphogluconate dehydrogenase ligand ... similarfashion to complex I, forms a dimer via the crystallo-graphic twofold axis. Two structures corresponding to complex III have been determined, ﬁrst using in-housediffraction data (IIIa) and then...

Tài liệu Báo cáo khoa học: Isochore structures in the chicken genome ppt

... theformer is only designed for genome analysis, whereas thelatter is a general one, suitable for econometrics and time-series analysis, etc.AcknowledgementsWe are grateful to the referees for ... cumulative GC proﬁle and the density distributionof CpG islands more and more accurately withdecreasing t0. On the other hand, a smaller t0leads to more segmentation points and shorter segmented ... chromosomes and sex chromosome Z, the threshold t0is set to 1000 to partition these chromosomes into compositionally dis-tinct domains. For microchromosomes, which aremuch smaller and contain...

Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc

... Trp1-Tat(1–9) to the EI complex was decreased.On the other hand, in the case of Ile3-Tat(1–9) theformation of the IEI complex was favored in comparison to the formation of the EI complex. Moreover, for ... site, forming ES,EI and IES complexes. The inhibitor is not able to bind to the competitive binding site. The IES complex is catalyti-cally inactive. The binding afﬁnity of substrate and inhibitor to ... give new insights for the developmentof more potent and speciﬁc peptide-based DP IV inhibitors.Such inhibitors could be useful for the treatment of auto-immune and inﬂammatory diseases.Keywords:...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... Pesticides and MPTP have also been found to be mitochondrial toxins. A recent report, however,suggests that mitochondrial complex I inhibition is notrequired for MPP+, and other pesticides, to induceneurodegeneration ... conversion of protoﬁbrils to ﬁbrillar structure.Thus, in the presence of MPTP, dopamine inducesa-synuclein to form ﬁbrillar structures which are prob-ably less cytotoxic than the protoﬁbrils. Similar ... MPTP, wasactually responsible for the neurotoxicity [7]. Inhumans and nonhuman primates, MPTP producesneurological, clinical and biochemical changes similar to those found in idiopathic Parkinson’s...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... (lanes 2 and 4) and 32P-labeled ETS-1(lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per-formed with EWS (lanes 2, 4 and 6) and 32P-labeled Htelo (lanes 3 and 4), dsHtelo (lanes 5 and ... found that EWS binds to G-quadruplex con-formations and not to single- and double-strandedconformations by RGG3, we aimed to determinewhether RGG3 of EWS modulates the formation orunwinding ... Protein–nucleic acid complexes. (A) EMSA was performedwith RGG3 (lanes 2 and 4) and 32P-labeled Htelo (lanes 1 and 2) orrHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes2 and 4) and 32P-labeled...

Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... high sensitivity and accuracy, the lectin microarray system is the best tool for a ‘cell proﬁler’, and it is expected to be applicable for selection of cancer-speciﬁc lectins and for qualitycontrol ... measure-ments, we built a customized qPCR array platform for glycogene expression proﬁling. The qPCR array con-sists of probes and primer sets for measuring 186 genemRNAs, and enabled the determination ... 120(RCA120), which binds to a ubiquitous N-glycan epi-tope. Each candidate was immunoprecipitated fromserum using commercially available antibodies (stageIV), and their glycan structures were proﬁled...

Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf

... developed to advise on the mini-mum requirements to follow in the storage and dis-semination of experimental data in ﬁelds such astranscriptomics and proteomics, which will ultimatelyallow data to ... uniﬁed tool, the kineticswizard.Data querying and visualization are provided by webbrowser interfaces for manual access and web services for automated access to both the newly developedMeMo-RK and ... ﬂexible programmatic access to the data, allowing users to write clients to customize and automate access directly from their simulationsoftware, systems biology platforms, tools or databases[25]....

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