... InsP6gradually into InsP5, InsP4,and the final product – Ins(2,4,6)P3and Ins(1,3,5)P3–via two alternative pathways [14].Bacterial BPPs containing two tandemly repeateddomains (dual domains) ... can increasethe amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efficient wayto improve the activity of the latter.IntroductionPhytate ... wasdetermined using the Bradford assay with BSA as thestandard [28].Phytase activity assayPhytase activity was determined by measuring the amount of phosphate released from InsP6using a modified...