Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
... Photochemical cross-linking of
Escherichia coli
Fpg protein to DNA
duplexes containing phenyl(trifluoromethyl)diazirine groups
Maria Taranenko
1
, ... as
compared to oxidized native DNA. The conditions for the
photochemical cross-linking of the reactive DNA duplexes
and the Fpg protein have been optimized to yield as high as
10% of the cross-linked .....
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... imagequant software. The
data were plotted as u (1 fraction of free DNA) versus the
protein concentration to determine the K
d
, which is equal to
the protein at which half of the free DNA is bound. ... 250
0.8
1
0.6
0.4
0.2
0
0.8
1
Fraction of RNA bound
Fraction of DNA bound
Fig. 4. Binding affinity of RGG3 to Htelo or
rHtelo. The DNA or RNA concentration was
fixed a...
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... experiments.
Protein- bound iron
FereneS was added in a final concentration of 20 mm to
the protein sample to give a total volume of 600 lL and a
protein concentration in the range 5–10 lm. The formation
of ... superoxide.
These proteins did not consume detectable amounts
of O
2
when one of the following substrates was
offered to a protein solution containing 7 lm meta...
Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
... analysis (tool to determine the
flux distribution in cellular networks, requires steady-state conditions); HPr, histidine -containing protein (component of the PTS); LacZ,
protein of the lactose degradation ... a regulatory FBA (rFBA) model of
catabolite repression with the o.d.e. model of Kremling
et al. [14] to predict intracellular fluxes of central
metabolism and gene exp...
Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... not
be confirmed to be otolin-1. Therefore, MALDI-TOF-
TOF tandem mass spectrometry was performed to
identify the proteins. The tryptic peptide mass finger-
printing spectra of these proteins were ... contrast, incubation with otolith extract
resulted in binding of a complex of the HMW aggregate containing
OMM-64 and otolin-1 to the beads.
H. Tohse et al. A novel protein in the...
Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf
... pK
a
value of 7.0, suggesting a single deprotonation step
of the catalytic water molecule to an hydroxide ion. The number of proton
transfers during the catalytic pathway was determined by monitoring ... formation of
a gem-diolate intermediate. The abstracted
proton is presumably transferred by the aci-
dic residue (Glu131) to the amine group of
the leaving peptide bringing to...
Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt
... mechanisms
of recognition and activation of interleukin-8 receptor
subtypes. J Biol Chem 274, 11768–11772.
18 Ji TH, Grossmann M & Ji I (1998) G protein- coupled
receptors. I. Diversity of receptor–ligand ... of Texas Medical Branch, Galveston, TX, USA
2 Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, USA
Chemokines constitute t...
Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
... library of LBAA
genomic DNA was screened for its ability to confer
tolerance to glyphosate. An ORF (EMBL Bank:
GU214711.1) of 1690 bp was isolated that encodes
GOX, an FAD -containing flavoprotein of ... stable
recombinant protein in E. coli at up to 4% of the
total soluble protein content of the cell [48].
Fig. 3. Microbial mechanisms of glyphosate
degradation. (A...
Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... of these proteins, a localization that
has been shown to be a critical factor for Ab-induced
toxicity [11]. In a transgenic mouse model of AD over-
expressing mutant human amyloid precursor protein,
tau-dependent ... disruption of the
association of these proteins could represent a poten-
tial therapeutic strategy for the treatment of AD. Here,
we used GST fusion proteins of F...
Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... coordinated to three nitrogen atoms
of His residues. The T2 copper was coordinated to
two nitrogens of His residues. On the basis of the elec-
tron density, we refined one oxygen atom (probably ... Y (2009) X-ray
structure of a two-domain type laccase: a missing link
in the evolution of multi-copper proteins. FEBS Lett
583, 1189–1195.
23 Lawton TJ, Sayavedra-Soto LA, Arp DJ &...
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