Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf

... Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing phenyl(trifluoromethyl)diazirine groups Maria Taranenko 1 , ... as compared to oxidized native DNA. The conditions for the photochemical cross-linking of the reactive DNA duplexes and the Fpg protein have been optimized to yield as high as 10% of the cross-linked .....

Ngày tải lên: 20/02/2014, 11:20

... imagequant software. The data were plotted as u (1 fraction of free DNA) versus the protein concentration to determine the K d , which is equal to the protein at which half of the free DNA is bound. ... 250 0.8 1 0.6 0.4 0.2 0 0.8 1 Fraction of RNA bound Fraction of DNA bound Fig. 4. Binding affinity of RGG3 to Htelo or rHtelo. The DNA or RNA concentration was fixed a...

Ngày tải lên: 15/02/2014, 01:20

... experiments. Protein- bound iron FereneS was added in a final concentration of 20 mm to the protein sample to give a total volume of 600 lL and a protein concentration in the range 5–10 lm. The formation of ... superoxide. These proteins did not consume detectable amounts of O 2 when one of the following substrates was offered to a protein solution containing 7 lm meta...

Ngày tải lên: 18/02/2014, 06:20

... analysis (tool to determine the flux distribution in cellular networks, requires steady-state conditions); HPr, histidine -containing protein (component of the PTS); LacZ, protein of the lactose degradation ... a regulatory FBA (rFBA) model of catabolite repression with the o.d.e. model of Kremling et al. [14] to predict intracellular fluxes of central metabolism and gene exp...

Ngày tải lên: 18/02/2014, 13:20

... not be confirmed to be otolin-1. Therefore, MALDI-TOF- TOF tandem mass spectrometry was performed to identify the proteins. The tryptic peptide mass finger- printing spectra of these proteins were ... contrast, incubation with otolith extract resulted in binding of a complex of the HMW aggregate containing OMM-64 and otolin-1 to the beads. H. Tohse et al. A novel protein in the...

Ngày tải lên: 18/02/2014, 17:20

... pK a value of 7.0, suggesting a single deprotonation step of the catalytic water molecule to an hydroxide ion. The number of proton transfers during the catalytic pathway was determined by monitoring ... formation of a gem-diolate intermediate. The abstracted proton is presumably transferred by the aci- dic residue (Glu131) to the amine group of the leaving peptide bringing to...

Ngày tải lên: 19/02/2014, 02:20

... mechanisms of recognition and activation of interleukin-8 receptor subtypes. J Biol Chem 274, 11768–11772. 18 Ji TH, Grossmann M & Ji I (1998) G protein- coupled receptors. I. Diversity of receptor–ligand ... of Texas Medical Branch, Galveston, TX, USA 2 Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, USA Chemokines constitute t...

Ngày tải lên: 19/02/2014, 05:20

... library of LBAA genomic DNA was screened for its ability to confer tolerance to glyphosate. An ORF (EMBL Bank: GU214711.1) of 1690 bp was isolated that encodes GOX, an FAD -containing flavoprotein of ... stable recombinant protein in E. coli at up to  4% of the total soluble protein content of the cell [48]. Fig. 3. Microbial mechanisms of glyphosate degradation. (A...

Ngày tải lên: 14/02/2014, 14:20

... of these proteins, a localization that has been shown to be a critical factor for Ab-induced toxicity [11]. In a transgenic mouse model of AD over- expressing mutant human amyloid precursor protein, tau-dependent ... disruption of the association of these proteins could represent a poten- tial therapeutic strategy for the treatment of AD. Here, we used GST fusion proteins of F...

Ngày tải lên: 14/02/2014, 14:20

... coordinated to three nitrogen atoms of His residues. The T2 copper was coordinated to two nitrogens of His residues. On the basis of the elec- tron density, we refined one oxygen atom (probably ... Y (2009) X-ray structure of a two-domain type laccase: a missing link in the evolution of multi-copper proteins. FEBS Lett 583, 1189–1195. 23 Lawton TJ, Sayavedra-Soto LA, Arp DJ &...

Ngày tải lên: 14/02/2014, 18:20