Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... alkyla- tion leading to a depletion of mtDNA in intact cells (Fig. 1). Here we report the synthesis and characterization of a novel mitochondria- targeted alkylating reagent and show that it alkylates ... should also alkylate mtDNA within mitochondria and cells. MitoDC-81 does not alkylate mtDNA in isolated mitochondria Having ascertained that mitoDC-81 was sequeste...

Ngày tải lên: 20/02/2014, 11:20

... molecular mass value of HAD was measured; instead of an adduct increase, or no change, the value had unexpectedly decreased from 22 417.0 Da to 22 413.2 Da, a loss of 4 Da. MS ⁄ MS of these molecular ... causes a 1 Da increase in any fragment ion containing that residue. The average mass gain of N-terminal and C-terminal fragment ions are plotted as positive and negative,...

Ngày tải lên: 18/02/2014, 16:20

... clipping of a fragment of desired length and sequence from a nat- ural RNA may be a useful application. For example, RNA fragments that involve modified nucleobases are easily obtainable from naturally ... this informa- tion is available, rational design has contributed to the development of new functional RNA, for example, sig- nalling aptamers and aptazymes [8–10]. Work in...

Ngày tải lên: 20/02/2014, 01:20

... Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells Anthoula Gaigneaux, Jean-Marie Ruysschaert and Erik Goormaghtigh Laboratory of Structure and ... The data were analysed with a window of five wavenumbers, assuming that adjacent wavenumbers are highly corr elated. A population o f 3 2 solutions was built at each generation, and...

Ngày tải lên: 21/02/2014, 03:20

... 213–222. 66 Hamaguchi M, Hamada D, Suzuki KN, Sakata I & Yanagihara I (2008) Molecular basis of actin reorgani- zation promoted by binding of enterohaemorrhagic Escherichia coli EspB to alpha-catenin. ... Y, Sagara H, Kabe Y, Azuma M, Kume K, Ogawa M, Nagai T, Gillespie PG, Sasakawa C & Handa H (2007) The enteropathogenic E. coli effector EspB facilitates microvillus effacing a...

Ngày tải lên: 14/02/2014, 22:20

... Sepuri, Sanjay Yadav, Hindupur K. Anandatheerthavarada and Narayan G. Avadhani Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA Cytochrome ... 86–93. 8 Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (1999) Dual targeting property of the N-terminal signal sequence of P450 1A1 . Targ...

Ngày tải lên: 18/02/2014, 16:20

... *SA*AAAAA* AGG AAA******* GAG ****AAA*** GGA *******AAA GAA ****AAAAAA AGA AAA****AAA AAG AAA*AAA*** GGD *******DDD GGE *******EEE GGL *******LLL GGN *******NNN GGS *******SSS GGP *******PPP A. J. Baldwin ... GGP precursor to the supernatant and the membrane frac- tions was similar to those of GGN, GGD, GGS, and AAG (Figs 2B and 4B). Interestingly, similar to the case of GGN...

Ngày tải lên: 19/02/2014, 07:20

... C-terminal regula- tory domain. These provide docking sites for downstream molecules and lead to the evasion of apoptosis, to proliferation, to invasion and to metas- tases, all of which are important ... for a 170kDa receptor tyrosine kinase. All ERBB proteins have four functional domains: an extracellular ligand-binding domain; a transmembrane domain; an intracellular ty...

Ngày tải lên: 16/02/2014, 09:20

... lung can- cer with epidermal growth factor receptor mutations. Br J Cancer 95, 998–1004. 16 Sutani A, Nagai Y, Udagawa K, Uchida Y, Koyama N, Murayama Y, Tanaka T, Miyazawa H, Nagata M, Kanazawa ... 3340–3346. 15 Asahina H, Yamazaki K, Kinoshita I, Sukoh N, Harada M, Yokouchi H, Ishida T, Ogura S, Kojima T, Okamoto Y et al. (2006) A phase II trial of gefitinib as first-line therapy for adv...

Ngày tải lên: 16/02/2014, 09:20

... Bishakha Shrestha, Saoni Mandal and other Mandal lab mem- bers for useful discussion and critical comments. This work was supported in parts by ARP (00365-0009- 2006) and American Heart Association ... gene activation A widely studied NR coactivator is activating signal cointegrator-2 (ASC2, also named AIB3, TRBP, TRAP250, NRC, NCOA6 and PRIP) [69]. ASC2 is a coactivator of mult...

Ngày tải lên: 16/02/2014, 14:20