Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

... form the phenolic substrate-binding site of the enzyme, whereas the T2 and the T3-pair coppers form the trinuclear site that is responsible for binding and reduction of the molecular oxygen. The ... Similarly, in the structure of TaLcc1, the last four amino acids (DSGL) penetrate inside the channel. This is known as a C-terminal plug or a C-terminal tail. On th...

Ngày tải lên: 14/02/2014, 18:20

... cargo binding sites, referred to as the major and minor binding sites [1]. These sites are located in the concave face of the protein near regions of invariant Trp and Asn arrays. The major binding site ... to the flexible importin-b binding domain. The importin-b binding domain is known to have an autoinhibitory function, whereby an internal NLS-like sequence competes fo...

Ngày tải lên: 14/02/2014, 19:20

... had an independent metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the ... the Mn or Fe ions incorporated into the enzyme as it accumulated in the E. coli cells. Indeed, the activity of the apo -enzyme was significantly lower than the...

Ngày tải lên: 14/02/2014, 22:20

... in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi- ate hydrolysis of ... domain than in the a domain [20]. This is also reflected in the rigidity of the protein. The atomic displacement factors of the PhyK structure are smaller for the...

Ngày tải lên: 16/02/2014, 09:20

... N-terminal SEA domain and the back site of the proteinase domain. This interaction would fix the SEA domain in a loca- tion on the opposite side of the proteinase domain from the active site cleft. ... site and are named according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-lo...

Ngày tải lên: 19/02/2014, 00:20

... role in the enzymatic reaction which will be discussed later. The structure of the active site, the type of protein ligands, the zinc-binding motif, the presence of a water Fig. 3. Structure of the ... residue in the zinc- bound MshB structure (PDB code 1Q74) adopts a sim- ilar strained conformation. On the other hand, in the absence of a zinc ion in...

Ngày tải lên: 19/02/2014, 00:20

... surfa- ces. Furthermore, the substrate-binding cleft of 1SH7 differs from that of 1IC6 and 1THM in shape, being seemingly deeper and more distinct, and in being more negatively charged than the binding ... ongoing mutagenic research in which single and combined amino acid substitutions aimed at increasing the stability of the Vibrio protein- ase are being tested. Eluci...

Ngày tải lên: 19/02/2014, 16:20

... respectively, in T. cruzi gGAPDH) were selected for the following reasons. (a) They are involved in the two phosphate–anion binding sites: Thr225 in the Pi site (for inorganic phosphate-binding site) and ... GAPDH structures available [16,18,19,22,24–29] and seems important to main- tain the correct positioning of the active residue Cys166 and the nicotinamide ring of...

Ngày tải lên: 20/02/2014, 02:21

... indolepyruvate, one step in the indolepyruvate path- way of biosynthesis of the plant hormone indole-3-acetic acid. The crystal structure of this enzyme from Enterobacter cloacae has been determined at 2.65 ... orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes. One of these, the hydrogen bond between the N1¢ at...

Ngày tải lên: 20/02/2014, 11:20

... crucial in determining the pK a of the his- tidine residue in the active site of RNases and conse- quently in determining the activity profile of this enzyme as a function of pH [26,27]. Another ... length of the N-terminal hairpin in HtA is intermediate between those in RNse T1 and a-sarcin, having 20 amino acids in HtA, 26 in a-sarcin and 12 in RNase T1....

Ngày tải lên: 18/02/2014, 08:20