Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... M1 3, M1 5, M1 7, M1 8, M1 9, M2 3, M2 4, M2 5, M2 6, M2 7, M2 8, M2 9, M3 0, M3 1, M3 4, M3 6, M3 8, M3 9, M4 0, M4 1, M4 6, M4 7, M4 8, M4 9, M5 1, M5 3, M5 4, M5 5, M5 7, M6 0, M6 2, M6 3, M6 6, M6 9, M7 1 a Measured at a bacterial ... Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial...

Ngày tải lên: 20/02/2014, 11:20

... The presence of two discrete populations of P2X 7 has been demonstrated in the plasma membrane of rat subman- dibular glands [14] and in mouse lymphoma cells [15], leading to the suggestion that P2X 7 may ... compilation ª 2007 FEBS 3021 among ‘raft’ and ‘nonraft’ domains of the plasma membrane. Lipid rafts are microdomains of the plasma membrane containing a high proportion...

Ngày tải lên: 19/02/2014, 00:20

... Ltd) and were cultured in serum- free Eagle’s minimum essential medium (Nissui Pharmaceu- tical Co., Ltd, Tokyo, Japan). Transfected cells were visual- ized by EGFP fluorescence and immunocytochemical staining ... of cultured hippocampal neurons. Fig. S4. Domain structure of MAP2 and the alignment of v-KIND-binding core (BD) region of MAP2 CD2 domain (702–744 aa) in human, mous...

Ngày tải lên: 14/02/2014, 19:20

... (C) and (D) to the right of the main peaks correspond to Na + adducts. Table 1. Metal to protein stoichiometries for CeMT-1 and CeMT-2 metalloforms determined by mass spectrometry and elemental analysis. ... 7400 7800 0 50 100 50 100 A B [–Met] [+Met] Cd 6 Cd 6 Zn 6 Zn 7 Cd 6 Zn Cd 8 Cd 7 Cd 9 [–Met] Mass (Da) Fig. 1. Deconvoluted ESI mass spectra of the various metalloforms...

Ngày tải lên: 16/02/2014, 15:20

... lM NBD-Cl, 50 lM MK571, 2 mM indomethacin, 2 mM probenecid, 10 mM vanadate or 20 nM concanamycin A. At the time points indicated by arrows, 5 lM TMRM was added. The TMRM fluorescence was monitored ... amount of rhodamine associ- ated with cells, cells were incubated with the dyes in a medium containing NaCl (132 mm), KCl (3.5 mm), CaCl 2 (1 mm), MgCl 2 (0.5 mm), glucose (10 mm), CCCP (1 l...

Ngày tải lên: 18/02/2014, 13:20

... chromatography using 200 m M imidazole. MW, molecular mass markers. (B) HPLC separation and fluorometric ana- lysis of the flavinic cofactor. Continuous line, cofactor extracted from purified recombinant ... presence of various electron acceptors, using spectrophotometric measurements at 340 nm. K m (lM) V max (nmolÆmin )1 Ælg )1 protein) Ferricyanide (1 m M)5 100 Q0 (100 l M) 3.5 1...

Ngày tải lên: 19/02/2014, 06:20

... T he enzyme was diluted to 2mgÆmL )1 in 50 m M Mes buffer, pH 7.0, containing 25 m M KCl and 2 m M dithiothreitol. An equal volume o f 1 M cysteine hydrochloride dissolved in the same buffer ... apo-phosphorylase formation was monitored by using a number of methods [17]: enzyme activity measurements using s amples taken from the incu- bation mixture; column sizing experiments t...

Ngày tải lên: 19/02/2014, 16:20

... presence of 10 mm Ca 2+ and < 30% of their activity without Ca 2+ (Fig. 3C,D). PhyH at 35 °C showed a spectrum (Fig. 4) with a weak minimum around 255 nm and a large positive maximum around 298 nm. ... other BPPs and HAP. Order of enzyme addition and reaction time Amount of liberated inorganic phosphate (lmol) Folds of increase in activityFirst enzyme Time (min) Second en...

Ngày tải lên: 14/02/2014, 15:20

... duplicated copy of dmrt1a on the Y chromosome was shown to be the dominant master regulator of male development [8], similar to Sry in mammals. Although many of the earliest cellular and morphological ... with Dmrt1 function studies in mice. The lack of dmrt1 in mutant mice caused a high incidence of teratomas and resulted in a failure of germ cells to arrest mitosis [...

Ngày tải lên: 14/02/2014, 19:20

... 1138–1146. 15 Krishnamoorthy L, Bess JW, Preston AB, Nagashima K & Mahal LK (2009) HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin. Nat Chem Biol 5, 244–250. 16 ... Nishimura S & Nishimura M (2007) Analysis of N-glycan in serum glycoproteins from db ⁄ db mice and humans with type 2 diabetes. Am J Physiol Endocrinol Metab 293, E1069–10...

Ngày tải lên: 16/02/2014, 08:20