Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

... regions containing over 20 grouped PS molecules). We have shown that doubling the PS concentration in the membrane results in an increase in the number of binding sites (compare Fig. 3D and 3E) and ... membrane-binding domain [9,10]. This Ca 2+ binding requires the presence of Gla residues. The Gla domains within the protein C family comprise 44 amino acids and contain...

Ngày tải lên: 18/02/2014, 06:20

... preincubating PDH120 with the inhibitory concentration of all these inhibitors prior to dilution in the unwinding reaction. Under these condi- tions, there was no inhibition of the unwinding ... polypeptide containing the properties of an antibiotic, intercalates into dsDNA and thereby inhibits nucleic acid synthesis [23]. Nogalamycin and daunorubicin are anthracycline antibio...

Ngày tải lên: 20/02/2014, 11:20

... turned inwards and interact with each other and with the a-helical linkers between the GAF A and B subdomains. In both structures, the cAMP-binding and cGMP- binding pockets are similar. A neighbouring ... GAF A and B bind cAMP (Fig. 1A,B,D,E). Again, the b1–b3 region is the major interaction site for the adenine moiety. In b1, an arginine residue (Ile417 in rP...

Ngày tải lên: 19/02/2014, 02:20

... mitochondrial proteins and ensures the elimination of damaged proteins [28]. This continuous Fig. 3. Determination of CML-protein content in liver mitochondrial matrix with aging. The CML content ... polyclonal antibody against Lon protease was raised in rabbit against a synthetic peptide corresponding to amino acids 208–221 of rat Lon. This antibody mainly recognized o...

Ngày tải lên: 20/02/2014, 11:20

... stabilization in normoxic conditions [62]. Interestingly, we observed that the insulin mRNA PTB binding site has an overlapping consen- sus CSD binding site. PTB, to date, has only been implicated in stability ... report here the identification of complexes, binding to the VEGF mRNA 5¢ -and3 ¢-UTR, that contain cold shock domain (CSD) and polypyrimidine tract binding (PTB) RNA bind...

Ngày tải lên: 19/02/2014, 12:20

... permeabilized and stained for F-actin with rhodamine-conju- gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the cells with either 10 ngÆmL )1 toxin ... response to aniso- mycin, suggesting that an intact actin cytoskeleton is also necessary for anisomycin signaling. Surprisingly, treatment of the cells with latrunc...

Ngày tải lên: 19/02/2014, 16:20

... seems that the scFvs fused to d1-EGFP are maintained in the cells by binding to antigen. Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular ... redu- cing conditions existing in cell compartments, which may prevent the formation of intradomain disulfide bonds within the VH and VL domains resulting in less stab...

Ngày tải lên: 19/02/2014, 18:20

... beneficial effects of glutamine; (a) by determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... Research Institute Maastricht (NUTRIM), Department of Human Biology, Maastricht University, the Netherlands Glutamine has an important function in the small intestine with respect t...

Ngày tải lên: 20/02/2014, 01:20

... different in distribution and in num- ber from that of the green fluorescent signals of the DePsipher dye seen in Fig. 1C and D, indicating that there is no interaction between DePsipher monomers and ... nuclear condensation and oligonucleosomal DNA laddering [19]. The hypothesis that mitochondria are associated with nuclear condensation and ⁄ or DNA degradation in...

Ngày tải lên: 20/02/2014, 03:20

... by insulin via IRS-1). ILK combines with an adaptor protein Nck2 and a five LIM domain- containing protein named PINCH (a particularly interesting new cysteine histidine protein) to form a ternary ... slightly. Cell adhesion to laminin was increased with the concen- trations of the coated laminin, and enhanced after insulin treatment at different concentrations of laminin. By con-...

Ngày tải lên: 21/02/2014, 00:20