Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

... A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells Julie L. Richards, Johanna R. Abend, Michelle L. Miller, Shikha Chakraborty-Sett, ... study, we used phage peptide display to identify a peptide, ATYSEFPGNLKP, that binds to CD40 and enhances adenoviral infection of mouse a...

Ngày tải lên: 20/02/2014, 11:20

... riboflavin biosynthetic pathway in plants is similar to that of eubacteria, rather than that in fungi and archaea. In view of the close similarity between puta- tive plant deaminases and their apparently ... archaea). We compare 2,5-diamino-6- ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases from the yeast Candida glabrata, the archaeaon Methanocaldococcus jannaschii and the eu...

Ngày tải lên: 18/02/2014, 18:20

... study. Name Sequence (residues 91–100) WT GGGAGGGGGG polyAla *SA*AAAAA* AGG AAA******* GAG ****AAA*** GGA *******AAA GAA ****AAAAAA AGA AAA****AAA AAG AAA*AAA*** GGD *******DDD GGE *******EEE GGL *******LLL GGN ... GAA and AGA mutants were prepared using a plasmid encoding GGA as a template and primers that anneal to nucleotide numbers 277–314 of psToc75. Plas- mids carrying seque...

Ngày tải lên: 19/02/2014, 07:20

... lysine methyltransferases. J Biol Chem 280, 5563–5570. 53 Qian C, Wang X, Manzur K, Sachchidanand, Farooq A, Zeng L, Wang R & Zhou MM (2006) Structural insights of the specificity and catalysis of a viral histone ... CREB-binding domain of CBP [31]. The KIX domain of CBP is a structural platform that is capable of binding several different families of transcriptional act...

Ngày tải lên: 16/02/2014, 14:20

... domains, a, b, b¢, and a , a linker region x between the b¢ and a domains, and a C-terminal acidic extension. The a and a domains contain the CGHC active site motif, and are sufficient alone to perform thiol–disulfide ... the effect of bacitracin in a variety of in vitro assays for PDI activity. On the basis of the BPTI refolding assay and peptide oxidation assay...

Ngày tải lên: 16/02/2014, 14:20

... to lm) and stabilize just one protein and thus are gener- ally protein and disease selective, if not specific. Many of the clinically important Fabry disease-asso- ciated a- galactosidase A variants ... intralysosomal accumulation of the enzymes substrate(s) [1,2]. Although each lysosomal storage disease has unique characteristics, generally they are progressive in nature and le...

Ngày tải lên: 18/02/2014, 16:20

... residue of a specific product causes a 1 Da increase in any fragment ion containing that residue. The average mass gain of N-terminal and C-terminal fragment ions are plotted as positive and negative, ... these fragment mass values originate from the same molecular ions, so they must all be characteristic of that protein’s sequence and molecular mass value. Thus, top-down d...

Ngày tải lên: 18/02/2014, 16:20

... The Research Council of Norway and Biotec Pharmacon ASA. References 1 Rao MB, Tanksale AM, Ghatge MS & Deshpande VV (1998) Molecular and biotechnological aspects of micro- bial proteases. Microbiol ... N-terminal pro domain is autocatalytically cleaved off when the enzyme has obtained its active conforma- tion, and the two C-terminal domains are cleaved off by heat treatment at...

Ngày tải lên: 19/02/2014, 07:20

... thaliana proteins also contain a PAC motif, and conversely that all PAC- annotated A. thaliana proteins contain a PAS domain. Therefore, in the case of A. thaliana,thePASandPAC motifs are inseparable, ... future. 3 Arabidopsis , Escherichia , Caenorhabditis and Azotobacter – a case study Some of the PAS domains have been analysed in detail. We chose four representative organis...

Ngày tải lên: 19/02/2014, 12:20

... recovered differentially based on varying stabilities. Remarkably, we have found that, at least in some circumstances, a quanti- tative correlation to biophysical data can be obtained from a statistical analysis of ... San Francisco, CA, USA To address questions of protein stability, researchers have increasingly turned to combinatorial approaches that permit the rapid analysis of l...

Ngày tải lên: 19/02/2014, 12:20