... Ipswich, MA). With the use of 0, 200, and 400 units of kPP, the samples were incubated for 30 min at 30 °C. The reactions were stopped with the addition of Laemmli buffer at 65 °C. The phosphoryla-tion ... orthologs of CorA, named Alr1 and Alr2, for Mg2+influx through the plasma membrane, and mostmembers of ascomycota appear to encode proteins of the this subfamily of CorA-related proteins. In the absence ... neartheir C-terminus and a GMN motif at the end of TM-A [11]. The short sequence connecting the twoTM domains appears to be oriented towards the out-side of the bacterial plasma membrane or the...