... performed inpositive reflector mode, collecting data from approximately3000 and 5000 single laser shots for MS and MS ⁄ MS anal-yses, respectively.Preparation of aggregate-free monomer for fibrillation ... applied to acarbon-coated formvar grid, left for 1 min, fixed with glu-taraldehye, wicked dry with filter paper, and 2% uranylacetate was added and the mixture was incubated for 2 min. The grid was ... filtrate from fractions eluted at 150–200 mmNaCl. For both Ab(M1–40) and Ab(M1–42), allmanipulations were performed at slightly alkaline pHto avoid the formation of structural contaminants pro-duced...