Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... 5473 Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima Leon D. Kluskens 1 , Gert-Jan W.M. van Alebeek 2 , Jasper Walther 1 , ... the first and only product on PGA and oligoGalpA. On the basis of its mode of action, PelB should be classified as an exo- polygalacturonase (EC 3.2.1.67...

Ngày tải lên: 20/02/2014, 03:20

... Construction of full- length xTRHR3 cDNA. A fragment of the receptor starting from the 5¢-end and ending in the middle of the transmembrane domain 6 was amplified using the specific primers, TRHR10 and ... copies of the TRH progenitor sequence, whereas only five TRH units are found in the rat and mouse [14,15], and six in humans [16]. The 5¢-flanking region of...

Ngày tải lên: 21/02/2014, 03:20

... absorbance values obtained with the cross-reacting and the immunogen peptides. Analysis of native VIH Direct ELISA on RP-HPLC fractions from the elution zone of CHHs and VIHs from lobster sinus glands ... terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles alo...

Ngày tải lên: 18/02/2014, 11:20

... itself against and interact with the membrane, thus enabling the SHBG domain to bind to the receptor on either the same or another cell. Axl, Sky and Mer in cancer The transforming activity of Axl under ... expands the boundaries of signalling mediators, and it is therefore not unlikely that Sky and Mer could be involved in Table 1. Affinities of Gas6 and protein S...

Ngày tải lên: 19/02/2014, 05:20

... model of the PDGF-C growth factor domain indicates the disulfide bridges in PDGF-C to consist of Cys250 and 294, Cys280 and 335, and Cys287 and 337, and the inter- monomeric bonds to consist of ... removal of the CUB domains and thereby activation of the growth factor domains. PDGF-C and -D contain both the CUB and growth factor domains when they are secre...

Ngày tải lên: 19/02/2014, 07:20

... using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were analyzed by ... at 150 rpm for 30 min, the reaction was stopped by adding an equal volume of 1.0 m HCl to the reaction mixture. The amount of product formed in the supernatant...

Ngày tải lên: 18/02/2014, 08:20

... indicate the M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) or absence (lanes ... concentration (IC 50 ) and median lethal dose (LD 50 ) values from HeLa cells and mice divided them into two subgroups: the more toxic forms (P I and P II) and th...

Ngày tải lên: 18/02/2014, 16:20

... characterized by the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produce adult tissues (i.e. the development of the gills and the production of an ... shell), which is accom- panied by significant growth of the soft body parts [21]. The mantle edge governs shell formation and body growth by the...

Ngày tải lên: 19/02/2014, 00:20

... and quantitative real-time RT-PCR, the locations of the primers DN)105 and DN)9 used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... structure in the nucleotide sequence of the 5¢-UTR in the transcripts starting from exons 1 and 1a, and found that the 5¢-UTR of the transcript from...

Ngày tải lên: 19/02/2014, 06:20

... with financial support from the Research Foundation of Raisiogroup (Raisio, Fin- land), the Finnish Funding Agency for Technology and Innovation (TEKES) and the Commission of the Euro- pean Communities, ... assay. Stereospecificity and substrate specificity The stereospecificity of T. reesei TYR2 was studied by following the activities on 15 mml-dopa, dl-dopa and d-dop...

Ngày tải lên: 19/02/2014, 06:20