Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt

... Si-face stereospecificity at C5 of coenzyme F 420 for F 420 H 2 oxidase from methanogenic Archaea as determined by mass spectrometry Henning Seedorf 1 ,Jo ¨ rg ... (1996) Si-face stereospecificity at C5 of coenzyme F 420 for F 420 -dependent glucose-6-phosphate dehydro- genase from Mycobacterium smegmatis and F 420 -depen- dent alcohol dehydrogena...

Ngày tải lên: 20/02/2014, 03:20

... weight of around 200 kDa (Fig. 7B). It was clear from the exchange pro- files (Fig. 5) that the TH8-TH9 region was involved in the formation of oligomers at pH 4.0. By contrast, dimer formation at ... deuterated T domain was prepared by incubation of the protein in D 2 Oat30°C for 6 h followed by concentration on a speed-vac. The cycle of incubation in D 2 O and concentra...

Ngày tải lên: 16/02/2014, 09:20

... infections. Abbreviations BGT, brassinin glucosyl transferase; BH, brassinin hydrolase; BHAb, brassinin hydrolase from A. brassicicola; BHLmL2, brassinin hydrolase from L. maculans; BO, brassinin oxidase; ... Brassinin saturation curve for BHLmL2 and (B) brassinin saturation curve for BHAb. The mixture was incubated at 23 °C for 45 min in the presence of increasing concentratio...

Ngày tải lên: 18/02/2014, 14:20

... labeling of the probes was chosen to detect RpCAbr mRNAs and RpCAtr mRNAs by green and red fluorescence, respectively. The antisense RpCAbr probe was synthesized by incorporation of DIG-conjugated UTP ... RpCAtr probe, streptavidin- horseradish peroxidase was added at a 1 : 100 concentration in TNB buffer. Slides were incubated for 2 h in a moist chamber at room temperature. Then...

Ngày tải lên: 18/02/2014, 16:20

... ultracentrifugation (160 000 g for 30 min), the crude extract was heated at 65 °C for 20 min, and the denatured proteins were removed by centrifugation (15 000 g for 30 min). The heat-resistant fraction was ... increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and then recordin...

Ngày tải lên: 18/02/2014, 18:20

... RNA helicase gene, was induced by low-K + , zeatin and cold treatments, and downregu- lated by high-K + stress. To further investigate the expression pattern of AtHELPS, pAtHELPS::GUS transgenic ... upregulat- ed by 100 lm K + ,2mm CsCl, zeatin and cold treat- ments, and downregulated by 100 mm K + and 200 mm NaCl treatments. Moreover, detailed analysis indicated that the expression...

Ngày tải lên: 14/02/2014, 18:20

... release (k cat,cbl-release ) for CN-Cbl was calculated to be 0.27 min )1 at 37 °C. From the initial rate of reactivation of the glycerol-inactivated holoenzyme, the rate constant of the reactivase ... the rate constant of the reactivase in cobalamin release (k cat,cbl-release ) for CN-Cbl was calculated to be 0.27 min )1 at 37 °C from the initial rate. The time course of...

Ngày tải lên: 15/02/2014, 01:20

... vector for silencing of TRAP1 expression (TRAP1-siRNA) was purchased from Shanghai GeneChem, Co. Ltd. The targeting sequence of the siRNA against rat TRAP1 was 5¢-CAACAGAGATTGATCAA AT- 3¢. A negative ... sequence of the homologue, TRAP1 was identified as a member of the HSP90 family. The ATPase activity of TRAP1 is inhibited by geldanamycin, which is a specific inhibitor of...

Ngày tải lên: 16/02/2014, 14:20

... incubation for 24, 48 and 72 h, the cells were incubated with 20 lL of MTT (at a final concentration of 0.5 mgÆmL )1 )at3 7°C for 4 h. The medium was removed and the precipitated formazan was dissolved ... the four pairs of gastric tissues showed great variance because of the different malignancies as well as individual differ- ences, the dysregulation of miR-23a in gastric...

Ngày tải lên: 18/02/2014, 04:20

... incubated at 37 °C for 4 h to allow removal of CMCT from U and G residues. After incubation, rRNA was precipitated and washed as described above. The pre- cipitate was dissolved in 20 lL of water ... of a 50% silica suspension in water was added and RNA was bound for additional 10 min at room temperature with gentle mixing. Silica was pelleted by cen- trifugation at 3000...

Ngày tải lên: 18/02/2014, 16:20