Tài liệu Báo cáo khoa học: Prevention of thermal inactivation and aggregation of lysozyme by polyamines docx

Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

... were separated by SDS/PAGE and stained by Coomassie brilliant blue. The twohemocyanin bands were cut out and dispersed. About100 lg of hemocyanin was used for the immunization of rabbits. For ... submitted to MALDI-TOF analysis and assigned to distinct subunits.Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin.About 10 lg of total hemolymph protein (HL) and 3 lgofpuriﬁedhemocyanin ... Gebauer and Markl [14] identiﬁed fourdistinct subunit types by means of immunological studies,native and denaturating PAGE. Our MALDI-TOF experi-ments and cDNA sequencing conﬁrm their results. By comparison...

Tài liệu Báo cáo khoa học: "Discourse Relations: A Structural and Presuppositional Account Using Lexicalised TAG*" docx

... (1998), Grote et al. (1997), Jayez and Rossari (1998) and Lagerwerf (1998). Secondly, the approach demands an understand- ing of the attentional characteristics of presupposi- tions. In particular, ... for NL understanding and/ or gener- ation. But the work to date surely shows the benefit of an approach that narrows the gap between dis- course syntax and semantics and that of the clause. ... comes from A substituting at the left-hand substitution site of y (in- dex 1) and B at the right-hand substitution site (in- dex 3). Semantically, the anchor of y ("because") asserts...

Tài liệu Báo cáo khoa học: Prevention of thermal inactivation and aggregation of lysozyme by polyamines docx

... Prevention of thermal inactivation and aggregation of lysozyme by polyamines Motonori Kudou1, Kentaro Shiraki1, Shinsuke Fujiwara2, Tadayuki Imanaka3 and Masahiro Takagi11School of ... Tm of lysozyme was 77.3 °C in the absence of additives. The addition of 100 mM polyamines and Argslightly increased the Tm of lysozyme by  1 °Cand0.5°C,respectively, whereas addition of ... beenpublished regarding the role of polyamines on thermal aggregation and inactivation of proteins.Materials and methodsMaterialsHen egg white lysozyme and betaine/HCl were purchasedfrom...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... wereidentiﬁed by the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated asthe number of apoptotic cells divided by the total number of cells counted. ... assembly and nucleocytoplasmictransport of newly synthesized pre-rRNAs [11].Because of its widespread distribution and broad range of involvement, as well as the cross-talking amongmolecules of ... impor-tant relationship among the regulation of nucleo-lin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative stress and Hsp70 overexpression. More importantly,...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: therapeutic aspects of vascular endothelial growth factor doc

... embryogenesis and adulthood, and functions by binding with two tyrosine kinase recep-tors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR ⁄ Flk-1),Fig. 1. The VEGF–VEGFR system and theinhibitors of various ... the Future’ of the Japan Society for thePromotion of Science, and the program ‘Promotion of Fundamental Research in Health Science’ of the Organi-zation for Pharmaceutical Safety and Research. ... here (Fig. 1), followed by discussion of two pathological conditions of angiogenesis in thebrain, stroke and brain tumors, along with possibletherapeutic strategies.VEGF and VEGFRsThe VEGF familyThe...

Tài liệu Báo cáo khoa học: Hypoxia-inducible factor-1a blocks differentiation of malignant gliomas pdf

... divergentrole and target genes of HIF-1a in different types of cancer.Malignant gliomas constitute a spectrum of braintumors with varying differentiation and malignancygrades, and with clinical ... theguidelines of the National Institutes of Health.Tissue samples and immunohistochemistryTumor tissues from a total of 95 adult patients, 53 men and 45 women (18 to 73 years of age; average ... signiﬁ-cant.AcknowledgementsWe thank Professor Ying Guo, Professor ChunkuiShao and Professor Jianyong Shao for providinghuman primary glioma tissues, and Professor HuilanRao for technical assistance...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... physiological role of this interaction is conserved [30,52]. Interactions of Vrp1p and Las17p in yeast and WIP and WASP-fam-ily proteins in mammalian cells are direct and resultin constitutive and apparently ... localization of Hof1p to the bud neck at elevated temperature and this in turn is important for cytokinesis and cellgrowth under these conditions.Interestingly, mutation of K485 and R486 in ... relativeexpression level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins.Lipid anchoring of C-Vrp1p bypasses therequirement for the LBD for endocytosis and growth at...

Tài liệu Báo cáo khoa học: Chemical approaches to mapping the function of post-translational modiﬁcations ppt

... proteolysis by incorporation of a variety of ligands onto proteaseSBL, including examples of natural PTMs such asbiotinylation and d-mannosylation (Fig. 3) [49]. Thependant ligands allowed ... UKIntroductionPost-translational modiﬁcations (PTMs) of proteinsmodulate protein activity and greatly expand the diver-sity and complexity of their biological function. Theubiquity of PTMs is reﬂected in their widespread ... code [5–7]. Asa consequence of the lack of direct genetic control of their biosynthesis, natural PTMs vary in site and level of incorporation, leading to mixtures of modiﬁed pro-teins that may...

Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... catabolic and signaling) in different cells and tissues [3,7].Several lines of evidence have suggested a key rolefor iPLA2-VIA in control of the levels of phosphatidyl-choline (PC) in cells by ... trend, peaking at G1 and lowest at G2⁄ M and lateS phase. Reduction of U937 cell proliferation by inhibition of iPLA2-VIAactivity was associated with arrest in G2⁄ M and S phases. The iPLA2-VIAeffects ... were found to be independent of the generation of free arachidonicacid or one of its oxygenated metabolites, and may work through regula-tion of the cellular level of phosphatidylcholine, a structural...

Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf

... astator and a rotor. The stator is formed by subunits a and b2, and the rotor is formed from multiple copies of subunit c. They form an oligomeric ring of non-covalently linked subunits, and rotation ... nFDlNaþwhere n is the number of translocated ions and F isthe Faraday constant.The c subunit of the eukaryal V1V0ATPases presentin organelles arose by duplication and fusion of thebacterial c subunit, ... mixture of 4 mL of solution A (0.1 m Tris ⁄ HCl,pH 6.8, 50 mg luminol in a total volume of 200 mL),400 lL of solution B (11 mg p-hydroxycoumaric acid in10 mL dimethylsulfoxide) and 1.2 lLofH2O2for...

Xem thêm