Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt

... 2003 The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 Hicham Bouabe* and ... NBD2 with the C-terminal segment of TAP1 confers the nucleotide binding behaviour of wild-type TAP1 whereas the chimeric NBD1...

Ngày tải lên: 20/02/2014, 02:21

... relative fluorescent units (RFU) and the standard deviation are plotted on the y-axis and the migration of protein standards of known molecular mass are shown above the relevant fractions. 2718 C. ... induction of apoptosis with the peak of the activities of the apoptotic caspases activity evident 24 h post exposure (data not shown). These data support a model...

Ngày tải lên: 19/02/2014, 13:20

... the axon terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonal tract [39,41]. The ... profile of an acetic acid extract of 30 lobster sinus glands. Only the part of the chromatogram where CHHs and VIHs are eluted is shown. The nature of th...

Ngày tải lên: 18/02/2014, 11:20

... determined by the Bradford method [37] using the Sigma prepared reagent and BSA as the standard. Effects of divalent metal ions and thiols on BH activities The effect of different metal cations on the ... 55% and 51% loss of the initial activities of BHLmL2 and BHAb, respectively. These results strongly suggest that Ser is involved in the catalytic activity of...

Ngày tải lên: 18/02/2014, 14:20

... CA sequences with one of the CA D. melanogaster sequences and, on the other hand, the other D. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano- gaster-3) ... approach of RpCAbr and RpCAtr, we cannot know the functional effect of changes of some key amino-acids. Origin and number of nonvertebrate CAs Although the...

Ngày tải lên: 18/02/2014, 16:20

... consistent with the NOE trend of the corresponding adjacent residues and hence does not conflict with the global interpretation of the data. With the exclusion of the N-terminal octapeptidyl and C-terminal ... interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE conn...

Ngày tải lên: 18/02/2014, 16:20

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure. How- ever, because the Zn-ste...

Ngày tải lên: 18/02/2014, 18:20

... regard- ing the phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those of the binding of I C to phospholipid ... studies on the membrane binding of I C and the disposition of the I C residues that are positively charged and make up the CPY -binding s...

Ngày tải lên: 19/02/2014, 05:20

... nucleotides of P7 are part of the G binding site, as are the terminal nucleotides of J6 ⁄ 7 and J8 ⁄ 7 [35–37]; the latter nucleotides are also not protected in the InDG b RNA. It should be noted that the ... the large P6 extension and the last three nucleotides (AU XG) of the intron. The sizes of the intron (InDG b ) and 5¢ exon (5E) are indicate...

Ngày tải lên: 19/02/2014, 07:20

... removal of the CUB domains and thereby activation of the growth factor domains. PDGF-C and -D contain both the CUB and growth factor domains when they are secreted and pro- teolytic cleavage is therefore ... on their activities points to a wide scope of biological effects; however further understanding of how these factors interplay with other members of the cyst...

Ngày tải lên: 19/02/2014, 07:20