Tài liệu Báo cáo khoa học: Tet repressor mutants with altered effector binding and allostery docx

... Spring Harbor, NY, USA. Altered TetR effector binding and allostery E M. Henßler et al. 4496 FEBS Journal 272 (2005) 4487–4496 ª 2005 FEBS Tet repressor mutants with altered effector binding and allostery Eva-Maria ... molecule. Equilibrium binding constants a ,(·10 7 M )1 ) [4-ddma-atc-Mg] + [atc-Mg] + TetR TetR i2 TetR i2 -V99N TetR i2 -L17A TetR TetR i2 TetR i...

Ngày tải lên: 20/02/2014, 01:20

... mM Hepes) and 23 °C with 0.3 M NaCl and 10 lML-ascorbate. In (B), the reactions were conducted under different pH conditions with 0.3 M NaCl, 10 lM L -ascorbate, and 10 lM CuCl 2 , and were incubated ... 25.8 and 53.7 °C. When evaluating the optimal design for a DNAzyme with respect to DNA-cleaving and RNA-cleaving activities, we found that a number of nucleotides within the...

Ngày tải lên: 16/02/2014, 15:20

... the last and first syllables in the reparandum and repair, respectively, and for normalized f0 and energy; results did not substantially differ from those presented here. port Levelt and Cutler's ... (Cooper and Sorenson, 1977; Gee and Grosjean, 1983; Selkirk, 1984), especially the relative inviolability of noun phrases (Wang and Hirschberg, 1992), and the length...

Ngày tải lên: 20/02/2014, 21:20

... in the germ cells (Gadus morhua [25] and Danio rerio [24]), whereas much broader and less restricted expression territories are seen within the tes- tis. With respect to a gonadal function of ... [20] and Oryzias latipes [21]) would infer a role correlated with Sertoli cell lineage specification and subsequently during testicular differentiation. The specific expression in spermatog...

Ngày tải lên: 14/02/2014, 19:20

... in Figs 1A and S1A, transiently expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal GAPDH) ... protein with N-terminal PGK and C-terminal cerulean) and cerulean–PGK (chimeric pro- tein with N-terminal cerulean and C-terminal PGK) were present throughout the cell...

Ngày tải lên: 16/02/2014, 09:20

... of the available data and mechanisms identified in leukemic stem and leukemic tumor cells and link this informa- tion with the known functions of mixed lineage leukemia and certain mixed lineage ... AF10 and DOT1L, and thus compete with their binding to the AF4 complex. An as yet unpublished study has demonstrated that the reciprocal AF4–MLL fusion protein retains its H3K4 HM...

Ngày tải lên: 16/02/2014, 14:20

... monitor the binding of the NarG peptides to NarJ. Surprisingly, the binding isotherm was biphasic, with the best fit obtained with a two binding site model, comprising a first site with binding stoichiome- try ... site with binding stoichiome- try (n) of 0.3 ± 0.2 and a binding constant (K d )of 3.4 ± 4 · 10 )9 m and a second with a stoichiometry of 0.7 ± 0.1 and a K...

Ngày tải lên: 16/02/2014, 14:20

... Metal binding stoichiometries and affinities were determined by ESI-MS and NMR, respectively. Both isoforms had equal zinc binding ability, but differed in their cadmium binding behaviour, with ... CeMT-1 and CeMT-2 if presented with Zn : Cd ratios as encountered by C. elegans. Using a Cd : Zn ratio of 33 : 1 [21 nm Cd(II) and 0.7 lm Zn(II)] and 0.1 lm of CeMT-1 and CeMT-...

Ngày tải lên: 16/02/2014, 15:20

... constructed three mutants: the T5S and T5A mutants, with the aim to examine the role of hydroxyl group; and the DT5 mutant [15]. Bioassay results showed that the T5A and DT5 mutants have almost ... cysteine-rich and metal -binding proteins [1]. In mammals, four subfamily MTs (i.e. MT1, MT2, MT3 and MT4), have been identified [2]. MT1 and MT2 are ubiquitous isoforms found in...

Ngày tải lên: 16/02/2014, 15:20

... [5] Reaction with NO Cysteine oxidation and Zn release Zn release is faster [38] Zn release is slower [38] Reaction with ROS Cysteine-oxidation and Zn-release rates relatively similar [38] Reaction with ... Cu 2+ ions through reduction to Cu(I) and binding to the protein. In this reaction, thiolate ligands are oxidized to disulfides concomitant with Zn 2+ release. The binding...

Ngày tải lên: 16/02/2014, 15:20