Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... 375–380. Functional regions of molluskan TnI H. Tanaka et al.4486 FEBS Journal 272 (2005) 4475–4486 ª 2005 FEBS Comparative studies on the functional roles of N- and C-terminal regions of molluskan and ... In the present study, we compared the functional roles of the N- and C-terminal regions of molluskan and vertebrate TnI and revealed for the ﬁrst time that (a) the alternative binding of the ... integrity of the tro-ponin complex. In molluskan muscles, the C-terminal region does not function and troponin regulatescontraction only through the activation exerted by the N-terminal region of...

Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... anin-line attack on the scissile phosphate in the DNAsubstrate. The role of the active-site magnesium ion isto stabilize the phosphoanion transition state and make a proton available for the ... characterization of the enzymes, we carried out ananalysis of the NaCl requirements and pH optimum of VsEndA and VcEndA. The optimum NaCl concen-trations for activity were found to be 425 mm forVsEndA and ... lower thermostability in cold-adaptedenzymes. The NaCl concentrations in which the ther-mal scans were performed mimic the physiological con-ditions that each of the enzymes face in their...

Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

... matrix-interactive factor. In the context of aggregation, the kinase activity of DNA-PK is essential for efﬁcient DNAend-joining. The massive and functional concentration of nucleoproteins on DNA in vitro ... that of nonbasic factors of DNA-PKcs, SAF-A and nucleolin, which were stablyconcentrated on anion-exchange resins (Experimentalprocedures). With regard to the action mode, the role of the N-terminal ... that the structural and func-tional distribution on relatively large proteins may beinvolved in the aggregation of aberrant DNAs.DNA-PK is the key factor for aggregation-coupledEJ The functional...

Tài liệu Báo cáo khoa học: "Chronometric Studies of Lexical Ambiguity Resolution " doc

... reading of the ambiguous word. In this way they are comparable co the syntactic contexts of the first experiment. The fourth experiment compared the use of non-priming contextual information in the ... person is much more likely to walk on the surface of a ship than on the surface of a pack of playing cards. Other types of contextual information can be brought to bear on ambiguity resolution ... basis of a model of ambiguity resolution in context, and at the same account for some of the contradictions in the existing literature. This work has three foci. The first is that we consider...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... embryo-genesis, all the evidence suggests that sex determinationmight disobey the conventional rules of evolutionaryconservation. The common picture emerging here is that the genes at the top of the cascade ... in the ﬁsh gonads The developmental expression of dmrt1 has been wellstudied in the context of gonadal induction and main-tenance, illuminating its important function. But whatabout the other ... deter-mination in ﬁsh came with the discovery of a dmrt1homologue on the Y chromosome of the ﬁsh medaka(Oryzias latipes). This Y-chromosomal gene is the product of a gene duplication of the autosomal...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... part in bothstructural and functional roles of NirF.Discussion On the basis of several criteria, including cell fraction-ation and the consequences of either deleting the putative signal sequence ... How-ever, the other catalytic site histidines (His 345 and His388) of NirS are not conserved in NirF.Restoration of denitriﬁcation upon complementation of the unmarked nirF deletion strain of P. ... stoichiometric,i.e. 1 mol of heme was taken up by 1 mol of NirF(concentrations of heme and protein were calculatedby using the extinction coefﬁcient mentioned in the experimental section and the standard Bradford...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... lost.Taken together, our results demonstrate an impor-tant relationship among the regulation of nucleo-lin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative ... the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... (2009)ATP-binding domain of heat shock protein 70 is essen-tial for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12...

Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding speciﬁcity pdf

... with the exception of residues 57–59 of one monomer, whichare part of a b-turn connecting two strands. Some of the strands present some kinks that break the continu-ity of the hydrogen bond ... sequence and of the 3D structure, theymust differ in their physiological function. This isconﬁrmed by the presence of a secretion signal at the N-terminus of HP1286 and E. coli YceI protein, and CBAFig. ... of the electron density clearly visible inside the barrel cav-ity of each monomer (Fig. 3A; see Fig. 3B for a scheme of the labeling system of the compound). Other contam-inants consisted of...

Tài liệu Báo cáo khoa học : Các phương thức thể hiện lời nói trên đài phát thanh docx

... bit diu the hiin bin sic ci nhin ro ring han Ndu nbu trong phuong thdc dgc, vai trd hd trg cua gigng dgc tdi hieu qua tdc phdm ttong nhidu the loai bao chi thudng md nhat, thi trong phuang ... thdng tin thyc te, thi ttong phuong thdc dgc, do bien dgng nhjp dieu thudng it. Nhip dieu the hien qua gigng dgc bao gid ciing dn djnh. Ngucd dgc cd the dgc vdn ban theo nhip dieu ddu nhanh, ... nhien, trong qud trinh the bien tren sdng, cd thd cd nhtrng thao tac xe djch nhdi djnh vd mat ngon tu. Tue la, qua trinh thd bien khong ddi bdi sy cbinh xdc tuyet ddi nbu trong van ban...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... containing mutations of the second or third miR-23a binding regions. (C) Similarly, overexpression of miR-23a failed to suppress the EGFP intensity in the reporter vector containing mutation of ... miR-23a on the expression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels.To conﬁrm the direct regulation ... adenocarcinoma. Both MTT and colony forma-tion assays conﬁrmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulatetheir targets by...

Xem thêm