Tài liệu Báo cáo khoa học: Second messenger function and the structure–activity relationship of cyclic adenosine diphosphoribose (cADPR) doc

... MINIREVIEW Second messenger function and the structure–activity relationship of cyclic adenosine diphosphoribose (cADPR) Andreas H. Guse University Medical Center Hamburg-Eppendorf, Center of Experimental ... between the base adenine (or hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands. These ether strands mimic t...

Ngày tải lên: 20/02/2014, 01:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... the area of the 11cROL and 13cROL peaks and expressed as the mean ± SD of three independent measurements. Retinal cell dissociation The zebrafish eyes were enucleated, and...

Ngày tải lên: 14/02/2014, 14:20

... 158(21) and 296(154) [14–17]. The high specific activity of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the ... catalytic efficiency, the overall functional defect of G372A- FVIIa was, if anything, smaller in the presence of TF, which suggests that the allosteric effect of...

Ngày tải lên: 18/02/2014, 08:20

... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the (s i , ... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at...

Ngày tải lên: 18/02/2014, 11:20

... structured within the plane of the membrane. Initial proteomics on the outer mem- brane from yeast mitochondria suggest that while most of the protein components are integral in the membrane, most of these ... related proteins from other fungi, or even in the fission yeast Schizosaccharomyces pombe. Both the lack of obvious orthologs in other fungi and the abundance...

Ngày tải lên: 19/02/2014, 07:20

... has Fig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-phosphopantothenoylcysteine (+ ... from D -pantothenate. The Dfp flavoprotein catalyzes the synthe- sis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopantothenate and cysteine usin...

Ngày tải lên: 19/02/2014, 12:20

... As MgP is also the product of the magnesium chelatase reaction, this emphasizes the importance of quantita- tive studies not only of the methyltransferase and chelatase, but also of the interaction ... stimulated. The discovery that another pro- tein, Gun4, can both stimulate Mg chelatase and bind MgP [12,25] adds another layer of complexity to both the regulation...

Ngày tải lên: 20/02/2014, 02:21

... entry of fatty acid into mitochon- dria. Thus, the steady-state level of malonyl-CoA in these tissues is determined by the relative activities of ACC and malonyl-CoA decarboxylase. Role of the ... ghrelin and adiponectin alter hypothalamic AMPK and malo- nyl-CoA levels [13,23,28,30–33]. The genetic evidence for the role of AMPK in the hypothalamus is less clear bec...

Ngày tải lên: 14/02/2014, 22:20

... genotype-directed therapy in this disease. KRAS mutation as a mechanism of resistance to EGFR-targeted therapy The KRAS protein is localized to the inner surface of the cell membrane. The binding of ligand ... reposition- ing of critical residues surrounding the ATP-binding cleft of the tyrosine kinase domain of the receptor and thereby stabilize the interaction...

Ngày tải lên: 16/02/2014, 09:20

... molecule takes the position of the adenine ring of ATP. The carbon and oxygen atoms of pantoate are shown in yellow and red, respectively. The carbon and oxygen atoms of ATP are shown in red, the nitrogen ... between the O2 atom of pantoate and the Nf atom of the side chain of Lys39. However, there are a large number of water-mediated networked interact...

Ngày tải lên: 16/02/2014, 09:20