Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding Zhengding Su 3 , Jiun-Ming Wu 1 , Huey-Jen Fang 1 , Tian-Yow Tsong 2,3 and ... state may be maintained mainly by key amino acids. In this study, two point-mutated proteins each with a single base substitution [alanine for tryptophan (...

Ngày tải lên: 20/02/2014, 01:20

... and K9, and of G129 with K110 and K133. Other charged amino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129. H M. Chen et al. Local stability and key acidic amino acids ... JZW, Fang H-J & Chen HM (2001) Signifi- cance of local electrostatic interactions in staphylococcal H M. Chen et al. Local stability and key acidic...

Ngày tải lên: 07/03/2014, 21:20

... corpus and the manual semantic role labeling it includes. Section 3 analyzes structural differences between verbatim and reconstructed text in the SSR as evaluated by a combination of manual and automatically ... downstream processes such as infor- mation extraction and question answering. Reli- ably identifying and assigning these roles to gram- matical text is an activ...

Ngày tải lên: 20/02/2014, 07:20

... advantage of intonational struc- ture in spoken sentence understanding in the combinatory categorial grammar formalism. (Bear & Price 1990) discusses integrating proso- dy and syntax in ... terms of available system functions. A dia- logue manager manages interaction with the speaker and retrieves database information, 3. PROSODY EXTRACTION As the input to t...

Ngày tải lên: 20/02/2014, 21:20

... [15,18]. The relative timing of steps involved in dimer formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor 5¢-phosphate group in the induction of ... taken after 20, 40 and 60 m in of incubation. The rates were calculated from linear plots of [P i ] released against the reaction time. W hen phosphate...

Ngày tải lên: 19/02/2014, 16:20

... the participating Table 1. Amino acid frequencies (%) in a- amylase linkers, in a set of globular proteins, in the Swiss-Prot databank and in intrinsically unstructured proteins. Amino acid Linkers a Globular proteins b Swiss-Prot c Intrinsically unstructured proteins b Ala ... has the theoretical capacity to disrupt a hydrophobic interaction in the linker...

Ngày tải lên: 14/02/2014, 18:20

... biotin and the target proteins, and thus allows their analysis by SDS ⁄ PAGE. Subcellular fractionation of porcine striatal brain tissue Porcine brain cut in half in the saggital plane was obtained fresh ... membrane staining of DAT and more diffuse cytoplasmic p2 5a staining. To better visualize the possible co-localization of p2 5a and DAT, the neurites indicated...

Ngày tải lên: 14/02/2014, 21:20

... cell migration in vitro and in avascular tissues, such as the cornea [15]. Transgenic mice expressing ANGPTL2 in adipose tissue show vascular in ammation and increased macrophage in ltration in adipose ... Oike Y, Yasunaga K, Hamada K, Miyata K, Matsumoto S, Sugano S, Tanihara H, Masuho Y & Suda T (2003) Inhibition of angiogenesis and vascular leakiness by angiopoietin...

Ngày tải lên: 14/02/2014, 22:20

... repeated twice; that is, if Ala25 of chain A is close to Asn77 of chain B, then Asn77 of chain A is close to Ala25 of chain B. Chain A Chain B Hydrogen bonds Ala25 Asn77, Arg80 AlaO–ArgNH1 AlaO–ArgND2 Asn26 ... structurally a subgroup of the lipocalin superfamily [15]. The prototype of lipocalins is reti- nol-binding protein (RBP), a protein of 182 amino acids pr...

Ngày tải lên: 16/02/2014, 14:20

... host–pathogen interactions and demonstrate a physiological role of the deubiquitinat- ing enzyme OTUB1 in Yersinia invasion. OTUB1 as a potential key player in regulating RhoA stability may represent a novel ... [12,13,17]. YpkA-mediated phosphorylation of Gaq impairs guanine nucleotide binding and subsequently inhibits Gaq-mediated signalling pathways including RhoA...

Ngày tải lên: 16/02/2014, 15:20