Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc

... 3949 The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 3 1 -helix, b-strand and random coil secondary structures Jens Danielsson, Ju ¨ ri Jarvet, Peter Damberg and ... residue. The bond lengths used were 1.33, 1.45 and 1.52 A ˚ and the fixed angles between the bonds were: 58°,64° and 75°. The rotations around the...

Ngày tải lên: 20/02/2014, 01:20

... enhancement was attributed to the physical prox- imity of the enzymes in the mini-cellulosomes and to cellulose targeting of the complexes via the CBM of the mini-scaffoldin [21]. The most active mini-cellulo- some ... con- trol sample and all the fractions, except for F1, which was found to be approximately fivefold more active than the others. The cellulosomes present...

Ngày tải lên: 18/02/2014, 08:20

... a-helical coiled -coil structures are known to mediate homo- and hetero-dimerization of proteins, and these sequences may facilitate the specific interactions between BAP31 and myosin heavy chain. Furthermore, ... in the BAP31 immunopre- cipitate, indicate that the association between BAP31 and c-actin was the result of specific interactions. Discussion We describe in thi...

Ngày tải lên: 20/02/2014, 23:20

... pH (6.0–8.0) and at low temperatures (0–35 °C). We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain ... (Fig. S1). There are two pairs of cysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residues are at the same positions a...

Ngày tải lên: 14/02/2014, 15:20

... indicated time points. The mixes were then incubated with [c- 32 P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the Coo- massie ... and (B) the top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the Coomassie Brilliant Blue-stained SDS ⁄ P...

Ngày tải lên: 14/02/2014, 19:20

... in the ChIP assays [60]. Similarly, ChIP experiments on the cyclin A, Cdc2 and cyclin B2 genes suggest that E2F4 and E2F6 bind to the promoters in G 0 and to some extent also in G 1 [55]. Furthermore, ... that the exact binding location is not identified in the experi- ments. Therefore, it is not clear whether the CDE ⁄ CHR is involved in binding these factors. Consistent wit...

Ngày tải lên: 16/02/2014, 09:20

... Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in either Keywords apoptosis; ... reagents and the recombinant human TRAIL ⁄ APO 2 ligand were purchased from Invitrogen and Feldan Bio (St-Laurent, QC, Canada), respectively. The caspase 3 substra...

Ngày tải lên: 16/02/2014, 09:20

... of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-rich sequence used primers SJS259 and SJS260, and ... SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introduced HindIII restriction sites and...

Ngày tải lên: 16/02/2014, 09:20

... isolated and analyzed by northern blot using Fluc and Rluc riboprobes. The radioactive signals were quantified by the STORM 820 equipment and IMAGEQUANT software (Molecular Dynamics). The Rluc ... [15]. On the other hand, other studies indicated that ASF ⁄ SF2 activates the translation of mRNAs bearing ASF ⁄ SF2 binding sites within the coding region in intact cells [16,54]....

Ngày tải lên: 16/02/2014, 15:20

... with thiol groups, and the cadmium speciation of the wild-type and the CeMT-2 knockout strain was distinctly different to the CeMT-1 and double knock- outs. Taken together, and supported by a simple ... bear the hallmarks of metallothioneins, i.e. they are small and cysteine-rich, and their expression is induced by metals [24]. More recently, RNA interference (RNAi) and...

Ngày tải lên: 16/02/2014, 15:20