Tài liệu Báo cáo khoa học: Enlarging the gas access channel to the active site renders the regulatory hydrogenase HupUV of Rhodobacter capsulatus O2 sensitive without affecting its transductory activity docx

... 77,7347–7351.O2sensitivity of the regulatory hydrogenase HupUV O. Duche´et al.3908 FEBS Journal 272 (2005) 3899–3908 ª 2005 FEBS Enlarging the gas access channel to the active site renders the regulatory hydrogenase ... In the case of the regulatory HupUV protein,our results favour the hypothesis of Volbeda et al.[25], which explains the O2resistance of RHs bylimited accessibility of the active site to ... pathways for gas access to the deeply buried active site [23–25]. As both molecular H2and O2are hydrophobic gases, they probably use the same access pathway to the hydrogenase active site. The amino-acid...

Tài liệu Báo cáo khoa học: a-Defensins increase lung ﬁbroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc

... the protein content of active ⁄ dephosphorylated b-catenin without changes intotal b-catenin, suggesting that a-defensins activate the b-catenin signaling pathway. We then studied the role of ... from granules of leukocytes and are implicated ininﬂammatory and ﬁbrotic lung diseases. In the present study, the effects of a-defensins on the proliferation and collagen synthesis of lung ﬁbroblastswere ... respectively, leading to inactivation of GSK3b and accumulation ⁄ activation of b-catenin [17,18]. To determine whether a-defensin-induced collagen synthesis and the accumulation ⁄ acti-vation of b-catenin...

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... initially to the a domain, thereby lead-ing to formation of the M4a cluster prior to formation of the M3b cluster, demonstrating that the individualbinding constants of divalent metal ions for the a ... of the a domain of rhMT-1a, showing the connectivities of the four divalent metal cations to the 11 cystei-nyl sulfurs. The numbering of the cysteines(1–11 starting from the N-terminus) and the four ... in efforts to elucidate the potentially cooper-ative nature of the metal-binding reaction within each of the domains. Thus, the goal is to elucidate the meta-llation mechanisms of the individual...

Tài liệu Báo cáo khoa học: Fermentative lifestyle in yeasts belonging to the Saccharomyces complex ppt

... NADHgeneration, due to amino acid biosynthesis. Much of the generation of NADH during amino acid biosyn-thesis takes place in the mitochondria. Because of the block in the respiratory chain caused by the addition of ... biosynthesis of sterols and fatty acids,heme ⁄ hemoproteins, NAD, and uracil [7,8]. The abil-ity to translocate ATP produced in the cytoplasm intomitochondria, and the ability to adjust the ... characteristic seems to bedetermined mainly by the activity of sugar carriers[5]. The inhibition of fermentation of glucose as wellas other sugars in the absence of oxygen has beendescribed as the Custer...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... Attempts to clarify the stoichiometry by adding a small amount of the puriﬁeddioxygenase to the reaction mixture with a large excess of the puriﬁed enzyme reported here to avoid the formation of 2,5-pyridinedicarboxylic ... time. The peak at 268 nm was assigned to 2,5-pyridinedicarboxylic acid based on the wavelength [10].Puriﬁcation and properties of the puriﬁed enzyme The activity of the enzyme present in the crude ... of 2 80 nm shifts to 265 nm if the protein contains a ﬂavin-type cofac tor [28]. We failed to identify the cofactor of the deaminase from strain 10dbecause the enzyme could not be puriﬁed in...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... the activity of the PTS system and therebyresult in further increases in hexose phosphate pools. The decreased LDH activity may contribute further to this effect by causing an accumulation of ... by the second gene in the las operon (Fig. 1).In order to obtain strains with increased PK activity anadditional copy of the pyk gene was recombined into the TP901-1 attachment site using the ... strain,CS1929 (37% PK activity) , thus contains only a singlepyk gene under the control of a synthetic promoter. The relative PFK activity in strain CS1929 was found to increase to over 160% of the wild-type...

Tài liệu Báo cáo khoa học: "Mining metalinguistic activity in corpora to create lexical resources using Information Extraction techniques: the MOP system" doc

... /'YES'@[102]. The different number of positions considered to the left and right of the markers in our training corpus, as well as the nature of the features selected (there are many more ... classification task.7 The nai-ve Bayes (NB) algorithm estimates the conditional probability of a set of features given a label, using the product of the probabilities of the individual features ... language, to use the termino-logy of logic where these concepts originate.3 Its constitutive markedness means that most of the times these sentences will have at least two indi-cators present,...

Tài liệu Báo cáo khoa học: "STRING-TREE CORRESPONDENCE GRAMMAR: A DECLARATIVE GRAMMAR FORMALISM FOR DEFINING THE CORRESPONDENCE BETWEEN STRINGS OF TERMS AND TREE STRUCTURES" pdf

... constraints )to design the structural representation for a given string of terms (because its topology is indepen- dent of the derivation tree of the grammar), as well as the nature of the correspondence ... indeed possible to change the label of node 2 to something else, or to move the node to the right of node 4, or even to exclude the node altogether. In short, the string-tree correspondence defined ... be possible thatZwe wish the topology oT the tree B to vary according to the choice of A., and this variation to be zn terms of the subtrees of the tree T For these reasons, we specify each...

Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf

... becomesavailable to the community in an accessible and utiliz-able form. Thus, the data ﬂow from the experiment to the consumer performing the analysis, the comparisonor the set-up of computer models ... (http://sabio.h -its. org/webservice.jsp) provide ﬂexible programmatic access to the data, allowing users to write clients to customizeand automate access directly from their simulationsoftware, systems ... and manipulating the results of the curve-ﬁtting algorithm. The left-hand panel allows the user to view eachassay in the data set and its automaticallyﬁtted initial rate. The red initial-rate...

Tài liệu Báo cáo khoa học: Pharmacologic chaperoning as a strategy to treat Gaucher disease ppt

... it to the lysosome. Once mutatedGC is localized to the lysosome, the glucosylceramidesubstrate is able to displace the inhibitor and allow the enzyme to turn over glucosylceramide, owing to Fig. ... binding to the folded mutantGC enzyme population in the ER, shift the equilibriumtowards folding allowing mutant GC to be trafﬁcked to the Golgi and on to the lysosome more efﬁciently,where the ... greater proportion of GC to be folded and thus engage the exo-cytic pathway that trafﬁcks it to through the Golgi and on to the lysosome, increasing the concentration of the folded enzyme hav-ing...

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