Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... 5¢-CAGCCGCCGTAGAA GTT GTAAGTCATCGCAAAG-3¢,5¢-CGCCGCCACCAGGG AA CATCCAGTCAATATCTAC-3, and 5¢-GCCGCCAC CAGGGAA TTGCCAGTCAATATCTAC-3¢, respectively. Conﬁrmation of the mutated nucleotides by automated sequencing ... case of the E315Q mutant, an additional faint band was also seen at an M r of  43 000. This band appeared as a degradation product during freezing and thawing of t...

Ngày tải lên : 20/02/2014, 01:20

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Tài liệu Báo cáo khoa học: How to remain nonfolded and pliable: the linkers in modular a-amylases as a case study ppt

... gigas (Mollusca, Bivalvia). Sequence data were depos- ited in GenBank (Table S1). Searches in databases Using the putative C-terminal domain of C. ﬂuminea as a query, sequence databases were searched ... genome databanks. Interestingly, this domain was found in some closely related bacterial species, but mainly in nonvertebrate animals, and invariably connected via a linker to an an...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... Interactions of Vrp1p and Las17p in yeast and WIP and WASP-fam- ily proteins in mammalian cells are direct and result in constitutive and apparently stable complexes [28,29,31,32]. In yeast, ... polyclonal GFP-spe- ciﬁc antiserum was a gift from J. Kahana and P. Silver (Dana Farber Cancer Center, Boston, MA). The anti-actin mAb was MAB1501 from Chemicon International (Teme...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... due to a widespread localization of both transcripts in the CNS and in peripheral organs. Materials and methods Animals Adult zebraﬁsh (Danio rerio) were purchased from local commercial sources. ... supporting cells located at the base of the neuromast and peripherally around the neuromast. A high level of prp2 transcripts was observed in kidney during zebraﬁsh embryonic an...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

... ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢ and 5¢-CGTTACATTATGCTCCATTGACTAACAACGA TG-3¢, were constructed based on fragment DNA sequences of the x-5 gliadin gene (GenBank accession numbers BE590673 and ... recombinant protein Sense (5¢-ATTTCATATGCAACAACAATTCCCCCAGC AACAATCA-3¢) and antisense (5¢-TCTCGGATCCTCA TAGGCCACTGATACTTATAACGTCGCTCCC-3¢) oligo- nucleotide primers having an initiation co...

Ngày tải lên : 20/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... Ozawa Y, Nakamura T, Kamata N, Yasujima D, Urushiyama A, Yamakura F, Ohmori D & Imai T (2005) Thermococcus profundus 2-ketoisovalerate ferredoxin oxidoreductase, a key enzyme in the archaeal energy-producing ... enzyme activity of the recombinant LDH was assayed at 70 ° C by monitoring the lactate-dependent NADH oxidation as the decrease in A 340 . The standard assay mixture co...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

... (panels E and J), are shown as ribbons (protein only, panels A E) and as surface (protein) and space-ﬁlling (carbohydrates and OG moieties) representations (panels F–J). The a IIb b 3 modules are ... Italy Introduction Integrins are heterodimeric transmembrane (TM) cellu- lar receptors involved in mechanical anchoring and two-way signaling [1]. Each a and b subunit has a...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

... The antibody detected YFP and YFP-K-Ras C14 as bands of 27 kDa and 27.5 kDa, respectively, and YFP-K-Ras full as a band of  55 kDa according to the expected molecular mass (Fig. 1A) . Membrane association ... authors thank the technical assistance of G. Schachner, S. Deza and C. Mas, and Eduardo Guimaraes (Departamento de Bioquı ´ mica-Instituto de Cieˆ ncias Ba ´ sicas...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... Colocalization of cystatin F with the lysosomal proteins LAMP-2 (Fig. 2A) and CD68 (Fig. 2B) revealed at least partial endosomal ⁄ lysosomal localization of cystatin F. Cathepsins are considered as ... originates from labeling with: (A) mouse anti-(LAMP-2) mAb and goat anti-mouse Alexa Fluorä 546-labeled secondary Ab; (B) mouse anti-CD68 mAb and goat anti-mouse Alexa Fluorä 54...

Ngày tải lên : 19/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... of sumoylation was greatly enhanced by cotransfection of PIASy, an E3 ligase for SUMO-1. The functional consequence of abolishing sumoylation was enhanced activation both of a transiently transfected reporter ... The indicated plasmids were transfected into HD11 cells and total RNA was isolated. Activation of the endogenous Myb-target gene, mim-1, was measured by real time P...

Ngày tải lên : 21/02/2014, 00:20

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