Tài liệu Báo cáo khoa học: Tachykinin-related peptide precursors in two cockroach species Molecular cloning and peptide expression in brain neurons and intestine docx

... HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein. In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and HepG2 cells ... short interfering RNA (siRNA) in human cells. Both HO-2 mRNA and protein are expressed in the eight human cancer cell lines examined, and HO-1 expression is d...

Ngày tải lên: 19/02/2014, 05:20

... l-xylulose in humans with pentosuria and have shown that aminopyrine, metyrapone and other xenobiotics cause an almost instantaneous increase in the conversion of UDP-glucuronate to glucuronate in isolated ... formation by antagon- izing the inhibitory effect exerted, presumably indirectly, by a combination of ATP-Mg and CoASH. C. L. Linster and E. Van Schaftingen Glucuronate fo...

Ngày tải lên: 19/02/2014, 07:20

... cells with PMA results in a strong increase in AP-1 binding to TREs [53]. Prm1- and Prm3- reporter gene expression and TPa and TPb mRNA expression are up-regulated by PMA in HEL cells [25]. While ... autocoids prostaglandin (PG) I 2 (prostacyclin) and nitric oxide (NO) through mechanisms involving direct cAMP- and cGMP- dependent protein kinase phosphorylation, respectivel...

Ngày tải lên: 19/02/2014, 16:20

... 2003 The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC Sandra J. Facey and Andreas Kuhn Institute of Microbiology and Molecular Biology, ... sensor kinase protein in the inner membrane of Escherichia coli containing four transmembrane regions. The periplasmic loops connecting the transmembrane regions are intriguingly shor...

Ngày tải lên: 20/02/2014, 11:20

... importin-b binding domain. The importin-b binding domain is known to have an autoinhibitory function, whereby an internal NLS-like sequence competes for the importin-a binding site, reducing binding ... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4 NLS peptide. The NLS peptide binds to the major binding site...

Ngày tải lên: 14/02/2014, 19:20

... FEBS 3759 Introduction C -peptide is derived from most of the proinsulin seg- ment in between the B and A chains of insulin [1] and has an important structural role in the proper folding and disulfide ... l M proinsulin C -peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C -peptide in the presence of insulin (C) and of...

Ngày tải lên: 18/02/2014, 04:20

... tightly and extend their acyl chains to maintain the integrity of the membrane. The substantial tilt (and resulting disorder) of the top leaf- let lipids and the slight increase in packing (and thus order) ... acyl chains, it presents an interesting insight into the interactions between asymmetrically-embedded peptides and nearby lipids. Similar to melittin, Ab40 is embedded asymmet...

Ngày tải lên: 18/02/2014, 08:20

... amino acid signal peptide and a 68 amino acid peptide belonging to the insulin family (Fig. 3C). If the signal peptide is eliminated and disulfide bridges are formed as in other insulin family peptides, ... growth factor-like peptide; BrdU, bromodeoxyuridine; DAPI, 4¢,6-diamidino-2-phenylindole; DILP, Drosophila insulin-like peptide; IGF, insulin-like growth factor; ILP, insulin-...

Ngày tải lên: 18/02/2014, 13:20

... : 2000 in blocking solution for 30 min. Cells were rinsed in NaCl ⁄ P i several times and incubated in blocking serum containing anti-mouse IgG (Vectastain) for a fur- ther 30 min. Staining was ... manufacturer’s instructions. Briefly, endogenous peroxidases were blocked in 0.3% H 2 O 2 , rinsed in NaCl ⁄ P i and incubated in blocking solu- tion for 20 min (Vectastain). Neuron...

Ngày tải lên: 18/02/2014, 13:20

... cysteine residues involved in intrachain disulfide bonding in AChEs are found in the sequence, only two members of the catalytic triad are present: serine and glutamate. The third residue, histidine, ... ColQ or PRiMA in the C. intestinalis genome. Molecular modeling of C. intestinalis AChE also indicates that the catalytic subunit can assemble into asymmetric forms Molecular m...

Ngày tải lên: 18/02/2014, 17:20