Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL rat liverplasma membrane vesicles or carnation petal ... Time course of inhibition of electrogenic BSP uptake into carnation petal microsomes by antibody A. The effect of [bilirubin].Experimental conditions: microsomes [2.6 mg proteinÆmL)1 in 0.25M ... vacuolarmembrane of plant cells. To this purpose, we investi-gated the presence of bilitranslocase in carnation petals and found a BSP uptake, inhibited by anti-bodies against bilitranslocase, in microsomal,...

Báo cáo khoa học: Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc

... activation of other kinases isgoverned by its cellular location. For example, in insu-lin-induced activation of PKB ⁄ Akt the insulin-inducedtransient increase in 3¢-phosphorylated inositide ... de Vries-Smits AM,van Lint J & Burgering BM (1998) Essential role forprotein kinase B (PKB) in insulin-induced glycogensynthase kinase 3 inactivation. Characterization of dominant-negative ... et al. Characterization and regulation of TSSK3 Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependentkinase-1-dependent signallingMarta...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... dimension. A sine function wasapplied in both dimensions of the time domain beforeFourier transformation.Integration of 1D and 2D signals was performed using1D winnmr and 2D winnmr software (Bruker, ... 1343where the relevant increase of m2produces the relevantintensity changes of ML ⁄ TG signals. In principle, suchan increase can be due to both a decrease in c1 and anincrease in p1after growth ... cross peak from the geminalprotons of carbons 1 and 3 of glycerol in TG [1].Cross peaks of lipids, including the T signal, wereabsent in cells characterized by low ML signals in 1Dspectra (Fig....

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... transcripts duringdevelopment and in adult tissuesTo gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression during ... gigas [5]. Interestingly, thisprotein, named C. gigas chitinase-like protein 1 (Cg-Clp1) was found to be involved in the control of growth and remodelling processes in a manner similarto its YKL-40 ... ﬁndingsargue for an early evolutionary origin and a high con-servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPs in humans and insects...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... indicate intron 6 of 75 bp in fragmentsB and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number atthe right of each RT-PCR ... results in no generation of protein isoforms.The 5¢-UTR of eukaryotic mRNA in uences the initi-ation step of protein synthesis and thereby in partdetermines the translational efﬁciency of the ... lines in RT-PCR ampliﬁedfragments A–J indicate regions that are removed by splicing,whereas a dashed line in exon 7 of fragment C represents a dele-tion of 39 bp. The thick lines indicate intron...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... whoseC-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s.ResultsThe C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of ... blue-lightactivation of PM H+-ATPase in guard cells of broadbeans involves protein kinase-mediated phosphoryla-tion of Ser and Thr residues in the C-terminus of thepump and 14-3-3 binding [17]. Much ... suggested by the parallel increase of the number of FC-bindingsites [18–20]. As to auxin, a soluble auxin receptor hasbeen reported to bind and activate the PM H+-ATPase,but the site of binding...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... bolded; N-terminalresidues of the catalytic domain are under-lined. The preregion is indicated in red, thepro-region in black, catalytic domain in blue and the C-terminal domains in violet. Theassumed ... on the stability of SPRK (3 lgÆmL)1) and PRK (7.5 lgÆmL)1) was determined by incubatingthe enzymes in 25 mm Hepes, 1 mm CaCl2pH 8.0 at 37 °C and 50 °C for 30 min containing various SDS ... 60, 90 and 120 min. At each time point, the samplewas incubated on ice for 5 min and remaining activitytoward Suc-AAPF-pNA was determined. One hundredpercent activity (0 min) is sample incubated...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... effective in protecting the enzyme from inactivation and its effect wasagain dose-dependent, suggesting that inhibition by DONoccurs at the active site or the glutamine-binding site of the enzyme ... thatpyridoxine may be the precursor of the pyrimidine moiety of thiamin in yeast is intriguing [27]. In fungi, pyroA and SOR1(PDX1), homologues of yeast SNZ1, play a defensive role against reactive ... organic chemicals includingamino acids, nucleic bases and vitamins were obtained fromcommercial sources. Acetylpyridine adenine dinucleotide(APAD) and 6-diazo-5-oxo-L-norleucine (DON) were...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... prostaglandin(PG) I2(prostacyclin) and nitric oxide (NO) throughmechanisms involving direct cAMP- and cGMP-dependent protein kinase phosphorylation, respectively, of TPa within its unique ... (TRE) and treatment of cultured cells with PMA results in astrong increase in AP-1 binding to TREs [53]. Prm1- and Prm3- reporter gene expression and TPa and TPbmRNA expression are up-regulated by ... dithiothreitol;8 lL) by heating at 95 °C for 2 min followed by slow cool-ing to room temperature for 30 min. The resulting double-stranded probes were then radiolabeled in 10 l L reactionscontaining 0.35...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... isolated recombinantCD11a and CD11b I domains. The effective inhibition of binding of ICAM-4 positive red cells by anti-ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells ... domains. The efﬁcient inhibition of theFig. 10. Speciﬁc binding of puriﬁed recombinant I domain GST fusionproteins to ICAM-4Fc in a solid phase assay. Dose-dependent binding of CD11a I domain ... competitor proteins and EDTA on binding of recombinant I domain GST to coated ICAM-4Fc in a solid phase assay. CD11a I domain GST (A) and CD11bI domain GST (B) binding to ICAM-4Fc was examined in the...

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