Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL rat liver plasma membrane vesicles or carnation petal ... Time course of inhibition of electrogenic BSP uptake into carnation petal microsomes by antibody A. The effect of [bilirubin]. Experimental conditions...

Ngày tải lên: 20/02/2014, 01:20

... activation of other kinases is governed by its cellular location. For example, in insu- lin-induced activation of PKB ⁄ Akt the insulin-induced transient increase in 3¢-phosphorylated inositide ... de Vries-Smits AM, van Lint J & Burgering BM (1998) Essential role for protein kinase B (PKB) in insulin-induced glycogen synthase kinase 3 inactivation. Characterization of d...

Ngày tải lên: 23/03/2014, 11:20

... dimension. A sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr and 2D winnmr software (Bruker, ... 1343 where the relevant increase of m 2 produces the relevant intensity changes of ML ⁄ TG signals. In principle, such an increase can be due to both a decrease in c 1 a...

Ngày tải lên: 18/02/2014, 13:20

... transcripts during development and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression during ... gigas [5]. Interestingly, this protein, named C. gigas chitinase-like protein 1 (Cg- Clp1) was found to be involved in the control of growth and remodelling processes i...

Ngày tải lên: 19/02/2014, 00:20

... indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of each RT-PCR ... results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part det...

Ngày tải lên: 19/02/2014, 06:20

... whose C-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of ... blue-light activation of PM H + -ATPase in guard cells of broad beans involves protein kinase-mediated phosphoryla- tion of Ser and Thr residues in the C-termin...

Ngày tải lên: 19/02/2014, 07:20

... bolded; N-terminal residues of the catalytic domain are under- lined. The preregion is indicated in red, the pro-region in black, catalytic domain in blue and the C-terminal domains in violet. The assumed ... on the stability of SPRK (3 lgÆmL )1 ) and PRK (7.5 lgÆmL )1 ) was determined by incubating the enzymes in 25 mm Hepes, 1 mm CaCl 2 pH 8.0 at 37 °C and 50 °C for...

Ngày tải lên: 19/02/2014, 07:20

... effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active site or the glutamine-binding site of the enzyme ... that pyridoxine may be the precursor of the pyrimidine moiety of thiamin in yeast is intriguing [27]. In fungi, pyroA and SOR1 (PDX1), homologues of yeast SNZ1, play a d...

Ngày tải lên: 19/02/2014, 12:20

... prostaglandin (PG) I 2 (prostacyclin) and nitric oxide (NO) through mechanisms involving direct cAMP- and cGMP- dependent protein kinase phosphorylation, respectively, of TPa within its unique ... (TRE) and treatment of cultured cells with PMA results in a strong increase in AP-1 binding to TREs [53]. Prm1- and Prm3- reporter gene expression and TPa and TPb mRNA expressi...

Ngày tải lên: 19/02/2014, 16:20

... isolated recombinant CD11a and CD11b I domains. The effective inhibition of binding of ICAM-4 positive red cells by anti-ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells ... domains. The efficient inhibition of the Fig. 10. Specific binding of purified recombinant I domain GST fusion proteins to ICAM-4Fc in a solid phase assay. Dose-dependent bin...

Ngày tải lên: 20/02/2014, 11:20