... in an inactive form as a monomer; in response to stress,it forms homotrimers that bind to the HSEs in thepromoter regions of hsp genes [19]. In addition, an in ammatory cytokine, interleukin-6, ... (Pierce, Rockford, IL, USA) at4 °C for 1 h. Bound fractions were washed five times withbinding buffer, and DNA-bound proteins were eluted withbinding buffer containing 1 mgÆmL)1biotin; this was ... N-terminal ATPase domain bindsADP ⁄ ATP, and can hydrolyze ATP; the centralb-sheet domain directly binds peptide substrates; andthe C-terminal a-helix domain regulates substratebinding [4–6].Hsp105a...