... generalunfolding, not leaving any hydrophobic cleft for bis-ANSbinding. High pressure is well known to denature proteins topartially folded states [15,17,38,48–50]. The large increase in bis-ANS binding ... sterically interfere with side-chain packing in theprotein’s interior. Mutations that create cavities in hydro-phobic environments generally cause proteins to becomeless stable [44,45]. Using the ... of microgram quantities of protein utilizing theprinciple of protein–dye binding. Anal. Biochem. 72, 248–254.27. Paladini, A.A. & Weber, G. (1981) Pressure-induced reversibledissociation...