Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf

... Quantitation of channeling in intact E. coli FEBS Journal 272 (2005) 3260–3269 ª 2005 FEBS 3269 Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli Georgia ... glycolytic intermediates to CO2 in intact Escherichia coli. Cellsincubated with [14C]glucose made14C-labeled glyco-lytic intermediates. When the incubation mix alsoincluded a [12C]intermediate, ... by theOPPP when making calculations of the fractionalimportance of channeled ﬂux in glycolysis.Experiments to investigate channeling of early glycolytic intermediates to CO2Figure 1 illustrates...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... N,N,N¢,N¢-tetramethyl-p-phenylenediamine [23,24].The use of a nonsaturating concentration of DCMU,an inhibitor known to close the PSII reaction center bybinding in the QBpocket and blocking electron transfer from QA to ... with saturating red actinic light from anarray of 655 nm light-emitting diodes at an intensity of 3000 lmol photons m)2ặs)1. Fluorescence was detected usinga PIN-photodiode after being passed ... emerge from a series of reactions leading to the full reduction of the quinone molecules locatedon the acceptor side of PSII. Previous work done usingqualitative or semiquantitative analysis of...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... protocols maydistort comparative immunoblotting data, it was of interest to re-examine the fate of cals equestrin by studying the entirecomplement of key Ca2+-binding elements in dystrophin-deﬁcient ... epresenting t he cytosolic Ca2+-binding component, calmodulin, was not changed in dystrophin-deﬁcient ﬁbres. The reduced 2D ÔStains-AllÕpattern o f luminal Ca2+-binding proteins in mdx prepara-tions ... ationic carbocyanine dye ÔStains-AllÕ was p er-formed in order to e valuate the fate of major Ca2+-bindingproteins in dystrophin-deﬁcient skeletal muscle ﬁbres. In contrast to a r elatively...

Tài liệu Báo cáo khoa học: "Structural Definition of Affixes from Multisyllable Words" docx

... definitions exclude admissible final strings from C2 or C3 for prefixes, and admissible initial strings from C2 or C3 for suffixes, in order to increase the reliability of the definition ... three single consonants that are beginning but not ending strings (J, S, V), and since again it takes two consonant strings to cause a sequence to be defined as an affix, this problem too can ... difficult in that the set of terminal consonant strings left after removing initial strings has more members that show a tendency to break internally. For example, breaks in the following strings...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... study of the role of recombination in mtDNA inheritance in S. cerevisiae showed that buddingcells were enriched in linear monomers of mtDNA. It wasproposed that in addition to a role in initiating ... theN-terminal helices 1 and 2 while leaving the interactinga-helix 8 intact, may destabilize the proteins leading to spurious protein–protein or/and protein–DNA interactions.The crystal structure of ... species.The role of Ydc2 for maintaining the content of mtDNA in ﬁssion yeast is not clear. Apart from DNA repair,recombination could be involved in the initiation of mtDNA replication (recombination-dependent...

Tài liệu Báo cáo khoa học: Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efﬁciency and Ca2+-sensitivity ppt

... rhodopsin contained 1.8 ng of GCAP-1 corresponding to a ratio of 1 : 1600 GCAP-1 to rhodopsin, ROSwith 15 lg rhodopsin contained 6.5 ng of GCAP-2 corresponding to aratio of 1 : 1340 GCAP-2 to ... in bovine rods in nearlyequal concentrations.Catalytic efﬁciency of ROS-GC1Previous determinations of the ratio of ROS-GC1 to rhodopsin in bovine ROS, based on the puriﬁcation of ROS-GC1 from ... substrate binding. In order to analyze data of a sigmoidal dependence in a linearLineweaver–Burk plot [41], we determined a Hill coefﬁcientn from a ﬁt of the direct plot. Values of Vmaxand...

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... activity and cytotoxicity of these enzymes [10]. The interest in ONC as a therapeuticagent has led to the expression of ONC recombinants,created using site-speciﬁc mutagenesis, in order to study ... determined using UV spectroscopy to measure the change in environment of the aromaticresidues during protein thermal unfolding. The proteinswere dissolved at 0.5 mgÆmL)1 in a buffer of 50 ... were grown at37 °C in DMEM, supplemented with 2 mML-glutamine,50 UÆmL)1penicillin, 50 UÆmL)1streptomycin and 10%fetal bovine serum, in a humidiﬁed incubator containing 5%CO2(v/v)....

Tài liệu Báo cáo khoa học: "Parameter Estimation for Probabilistic Finite-State Transducers∗" doc

... such as deter-minization and minimization of transducers).This programming paradigm supports efﬁcientnondeterminism, including parallel processing over in nite sets of input strings, and even ... ex-treme, if each input string is fully observed (not the case if theinput is bound by composition to the output of a one -to- manyFST), one can succeed by restricting g to each input string in turn; ... replaced by binary operations⊗ and ⊕ on K. Thus ⊗ is used to combine arcweights into a path weight and ⊕ is used to com-bine the weights of alternative paths. To sum over in nite sets of cyclic...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... accompanied by improved bind-ing to longer ligands (up to ﬁve atoms in the mainchain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 from native GLYAT (Fig. ... bind in acondensed, high-energy and noninhibitory conforma-tion. Glyphosate sensitivity is partly restored by muta-tion of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory ... [27] and G96A in EPSPS from Klebsi-ella pneumoniae [28]. The G96A variant from E. coli ishighly resistant to glyphosate, owing to the methylgroup protruding into the glyphosate-binding site [29];however,...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... domain[20,21]. The SH3 domain of Fyn binds to proline-richmotifs within the sequence PXXP in interacting pro-teins. Seven such motifs are present in the proline-richdomain of the longest isoform ... thattau interacts with Fyn-SH2. Proteins harbouring SH2domains bind to phosphorylated tyrosines on their tar-get proteins, thereby coupling the activity of tyrosinekinases with intracellular ... neuronal responses to neuro-toxic insults such as Ab remains to be determined. Theuse of mutant tau constructs in which individual tyro-sines were mutated to phenylalanine allowed us to demonstrate...

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