Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf

... Quantitation of channeling in intact E. coli FEBS Journal 272 (2005) 3260–3269 ª 2005 FEBS 3269 Quantitative estimation of channeling from early glycolytic intermediates to CO 2 in intact Escherichia coli Georgia ... glycolytic intermediates to CO 2 in intact Escherichia coli. Cells incubated with [ 14 C]glucose made 14 C-labeled glyco- lytic...

Ngày tải lên: 19/02/2014, 18:20

... N,N,N¢,N¢-tetramethyl- p-phenylenediamine [23,24]. The use of a nonsaturating concentration of DCMU, an inhibitor known to close the PSII reaction center by binding in the Q B pocket and blocking electron transfer from Q A to ... with saturating red actinic light from an array of 655 nm light-emitting diodes at an intensity of 3000 lmol photons m )2 ặs )1 . Fluorescence was...

Ngày tải lên: 19/02/2014, 05:20

... protocols may distort comparative immunoblotting data, it was of interest to re-examine the fate of cals equestrin by studying the entire complement of key Ca 2+ -binding elements in dystrophin- deficient ... epresenting t he cytosolic Ca 2+ - binding component, calmodulin, was not changed in dystrophin-deficient fibres. The reduced 2D ÔStains-AllÕ pattern o f luminal Ca 2+ -binding...

Ngày tải lên: 19/02/2014, 16:20

... definitions exclude admissible final strings from C 2 or C 3 for prefixes, and admissible initial strings from C 2 or C 3 for suffixes, in order to increase the reliability of the definition ... three single consonants that are beginning but not ending strings (J, S, V), and since again it takes two consonant strings to cause a sequence to be defined as an affix, this...

Ngày tải lên: 19/02/2014, 19:20

... study of the role of recombination in mtDNA inheritance in S. cerevisiae showed that budding cells were enriched in linear monomers of mtDNA. It was proposed that in addition to a role in initiating ... the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein–protein or/and protein–DNA interact...

Ngày tải lên: 20/02/2014, 11:20

... rhodopsin contained 1.8 ng of GCAP- 1 corresponding to a ratio of 1 : 1600 GCAP-1 to rhodopsin, ROS with 15 lg rhodopsin contained 6.5 ng of GCAP-2 corresponding to a ratio of 1 : 1340 GCAP-2 to ... in bovine rods in nearly equal concentrations. Catalytic efficiency of ROS-GC1 Previous determinations of the ratio of ROS-GC1 to rhodopsin in bovine ROS, based on the...

Ngày tải lên: 21/02/2014, 00:20

... activity and cytotoxicity of these enzymes [10]. The interest in ONC as a therapeutic agent has led to the expression of ONC recombinants, created using site-specific mutagenesis, in order to study ... determined using UV spectroscopy to measure the change in environment of the aromatic residues during protein thermal unfolding. The proteins were dissolved at 0.5 mgÆmL )1 in...

Ngày tải lên: 19/02/2014, 12:20

... such as deter- minization and minimization of transducers). This programming paradigm supports efficient nondeterminism, including parallel processing over in nite sets of input strings, and even ... ex- treme, if each input string is fully observed (not the case if the input is bound by composition to the output of a one -to- many FST), one can succeed by restricting g to each inpu...

Ngày tải lên: 20/02/2014, 21:20

... accompanied by improved bind- ing to longer ligands (up to five atoms in the main chain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 from native GLYAT (Fig. ... bind in a condensed, high-energy and noninhibitory conforma- tion. Glyphosate sensitivity is partly restored by muta- tion of Ala100 to glycine, allowing glyphosate to...

Ngày tải lên: 14/02/2014, 14:20

... domain [20,21]. The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform ... that tau interacts with Fyn-SH2. Proteins harbouring SH2 domains bind to phosphorylated tyrosines on their tar- get proteins, thereby coupling the activity of tyrosine kinases wi...

Ngày tải lên: 14/02/2014, 14:20