Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase Ina Catrein, Richard Herrmann, Armin Bosserhoff ... N-terminusinstead of a free amino group [32]. Therefore, theasparagine in position 26 was labeled within the intactprotein at its a- amino group and represents the aminoterminal amino acid of P40. ... cell viability in all bacteria analyzed [26].To test experimentally whether there is a type I SPase activity in M. pneumoniae, we determined theN-terminus of P40 as it appears in protein extracts...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... complexes. This multimerization caninvolve either of the PDZ domains, as for example in glutamate receptor interacting protein 1 (GRIP1) [55]or inactivation no afterpotential D (INAD) protein[56]. ... remodelling is a crucialprocess occurring during cell migration and invasion in various physiological (i. e. embryonic development,ovulation, angiogenesis, wound healing) and patho-logical processes, ... whether it comprises a more generalmechanism for furin-mediated activation of transmem-brane substrates. The activation of ADAM17 by furin[68] and our observation of an interaction betweenGRASP55...

Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx

... reoxygenation but is ineffective atmaintaining glutathione levels during hypoxiaGlutathione is one of the main antioxidant compounds in the cell, and it is also essential for DNA synthesisand repair ... this a- keto acid isassociated with protective effects against hypoxia andreoxygenation. This is mainly ascribed to its ability tomaintain redox status [17], intervening in the DNArepair system ... The increased uptake of pyruvate might allowHepG2 cells to maintain their antioxidative capacities in a way independent of glutathione during hypoxia.However, after reoxygenation, the beneﬁcial...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... activity N-acetyl-Leu-Glu-His-Asp-pNA No activity No activity No activity N-acetyl-Val-Glu-Ile-Asp-pNA No activity No activity No activity N-acetyl-Ile-Glu-Thr-Asp-pNA No activity No activity No activity IC50 in nM for ... K) for papain and ervatamin -A alsoreveal that side chain conformations of the S2 subsiteresidues are less ﬂexible in papain than in ervatamin -A. The distribution of the chi2 dihedral angle of ... FEBSStructural insights into the substrate speciﬁcity and activity of ervatamins, the papain -like cysteine proteasesfrom a tropical plant, Ervatamia coronariaRaka Ghosh, Sibani Chakraborty, Chandana...

Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

... USA) for expression as a Gal4 DNA-binding domain fusion protein. This bait wastransformed into an AH 109 yeast strain and used to screen a human brain two-hybrid cDNA library from Clonetechas ... proteinsare of particular relevance from the clinical point of view. Anp32 proteins are powerful inhibitors of phos-phatase 2A (PP 2A) , a major serine ⁄ threonine phospha-tase involved in many ... structure in solution of theevolutionarily conserved N-terminal leucine-rich repeat (LRR) domain andmodeled its interactions with other proteins, taking PP 2A as a paradig-matic example. The interaction...

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... equal to those for maltooligosaccharides. Despite having similar folds, the sugar-binding site of the N-domain part of TvuCMBP and other bacterial sugar-binding pro-teins are strikingly different. ... critical in determining the speciﬁcity of the bacterial sugar-binding proteins.Evaluation of the binding afﬁnities byﬂuorescence measurementsThe Kdvalues of TvuCMBP for binding of sugars ... maltodextrin-binding protein ( TvuCMBP).The bacterial sugar-binding protein is a member of the bacterial ATP-binding cassette transport system,and the mechanism of the maltodextrin transport in Escherichia...

Tài liệu Báo cáo khoa học: "Dependency Hashing for n-best CCG Parsing" doc

... derivationand in a variety of orders. This means that compar-ing all of the dependencies in a particular state mayrequire traversing the entire sub-derivation at thatpoint. Parsing is already ... Curran, 2004b).2.3 n-best parsing and rerankingMost parsers use dynamic programming, discard-ing infeasible states in order to maintain tractability.However, constructing an n-best list requires ... n-best candidates:Constraint. At any point in the derivation, any n-best candidate must not have the same dependenciesas any candidate already in the list.Avg P/sent Distinct P/sent % Distinct10-best...

Tài liệu Báo cáo khoa học: "Topic Models for Dynamic Translation Model Adaptation" pptx

... suitability of the available parallel trainingdata. Domains (e.g., newswire vs. blogs) may varywidely in their lexical choices and stylistic prefer-ences, and what may be preferable in a general ... selecting rele-vant phrases for translation. Matsoukas et al. (2009)introduced assigning a pair of binary features toeach training sentence, indicating sentences’ genreand collection as a way ... training data, weabstract the notion of domain to include automati-cally derived subcorpora with probabilistic member-ship. This topic model infers the topic distribution of a test set and...

Tài liệu Báo cáo khoa học: Enlarging the gas access channel to the active site renders the regulatory hydrogenase HupUV of Rhodobacter capsulatus O2 sensitive without affecting its transductory activity docx

... with variation of less than 15%.ProteinsAerobiosis Anaerobiosis Anaerobiosis + MV+ Activity HDformationH2formation Activity HDformationH2formation Activity HDformationH2formationHupUV ... HupUVsensitive to O2inactivation. Also, in contrast with the wild -type protein,the mutated protein exhibited an increase in hydrogenase activity afterreductive activation in the presence of reduced ... it will bepossible to design a hydrogenase that exhibits high activity together with O2insensitivity. Experimental procedures Bacterial strains and plasmidsThe strains and plasmids used in...

Tài liệu Báo cáo khoa học: Kinetic basis for linking the ﬁrst two enzymes of chlorophyll biosynthesis doc

... ChlM catalysisby accelerating the formation and breakdown of thecatalytic intermediate, providing a kinetic link betweenthe ﬁrst two reactions of chlorophyll biosynthesis, withthe signalling ... of the intermediate occursmuch earlier (Fig. 3B), and intermediate accumulationoccurs within the 2 ms dead time of the instrument.This dramatic acceleration in formation of the interme-diate ... MgP as the common factor.Interactions between the methyltransferase and mag-nesium chelatase are likely to be crucial in determiningthe availability of MgP for both signalling [20] andbiosynthetic...

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